PMID: 26767113Sep 1, 2014Paper

Evidence of Energy Transfer from Tryptophan to BSA/HSA Protected Gold Nanoclusters

Methods and Applications in Fluorescence
Sangram RautIgnacy Gryczynski

Abstract

This work reports on the chromophores interactions within protein-protected gold nanoclusters. We conducted spectroscopic studies of fluorescence emissions originated from gold nanoclusters and intrinsic tryptophan (Trp) in BSA or HSA proteins. Both, steady state fluorescence and lifetime measurements show a significant Forster resonance energy transfer (FRET) from Trp to the gold nanocluster. Tryptophan lifetimes in the case of protein-protected gold nanoclusters are 2.6ns and 2.3ns for BSA and HSA Au clusters while 5.8ns for native BSA and 5.6 for native HSA. The apparent distances from Trp to gold nanocluster emission center, we estimated as 24.75A0 for BSA and 23.80A0 for HSA. We also studied a potassium iodide (KI) quenching of protein-protected gold nanoclusters and compared with the quenching of BSA and HAS alone. The rates of Trp quenching were smaller in BSA-Au and HSA-Au nanoclusters than in the case of free proteins, which is consistent with shorter lifetime of quenched Trp(s) and lower accessibility for KI. While Trp residues were quenched by KI, the emissions originated from nanoclusters were practically unquenched. In summary, for BSA and HSA Au clusters, we found 55% and 59% energy transfer efficiency respectivel...Continue Reading

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Citations

Sep 27, 2018·Scientific Reports·Mathieu OuelletteÉlodie Boisselier
Feb 14, 2019·Physical Chemistry Chemical Physics : PCCP·Ben A RussellPaul A Mulheran
Apr 24, 2015·Physical Chemistry Chemical Physics : PCCP·A Kyrychenko
Mar 7, 2019·Chemical Society Reviews·Xi Kang, Manzhou Zhu
Mar 19, 2020·Journal of Fluorescence·Uchangi Satyaprasad AkshathSridevi Annapurna Singh
Feb 21, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Nora AlkudaisiYu Chen
Nov 14, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·B A RussellY Chen
Apr 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yu-Chen HsuShu-Hui Chen
Sep 8, 2019·The Protein Journal·R P DasK I Priyadarsini
Jun 7, 2017·Chemical Reviews·Indranath Chakraborty, Thalappil Pradeep

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