PMID: 3745951Mar 1, 1986Paper

Evidence of mouse epidermal subpopulations with different cell cycle times

The Journal of Investigative Dermatology
O P ClausenA Cromarty

Abstract

In order to obtain information on the distribution of total cell cycle times in hairless mouse epidermis, basal cells were isolated and prepared for DNA flow cytometry at intervals after a pulse labeling with 50 microCi of thymidine. The DNA distributions were recorded, and cells were sorted from windows in the S, G2, and G1 phases of the cell cycle, collected on glass slides, and subjected to autoradiography. The proportions of labeled cells were scored in each fraction, and the percentage of labeled mitoses was determined in histologic sections from the same animals. Grain count distributions were recorded at selected time points over labeled cells in sorted fractions and over labeled mitoses. The movement of the labeled S-phase cohort was thus followed through all cell cycle phases. Peaks in labeled cells were observed at about 36 h in S phase, G2 phase, and mitosis, and high levels of labeled G2 cells and mitoses were seen at about 80 h. These results indicate the existence of one rapidly cycling subpopulation of keratinocytes with a cell cycle time slightly less than 30 h, in addition to keratinocytes with considerably longer cell cycle times. The first peak of labeled G2 cells reached only about 30%. This is consistent wi...Continue Reading

Citations

May 1, 1993·Cell Proliferation·E AarnaesP De Angelis
Jan 1, 1988·Annals of the New York Academy of Sciences·K Elgjo, K L Reichelt
Nov 1, 1992·Journal of Dermatological Science·Y HashimotoH Iizuka

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