Evidence of syntrophic acetate oxidation by Spirochaetes during anaerobic methane production

Bioresource Technology
Sang-Hoon LeeHee-Deung Park

Abstract

To search for evidence of syntrophic acetate oxidation by cluster II Spirochaetes with hydrogenotrophic methanogens, batch reactors seeded with five different anaerobic sludge samples supplemented with acetate as the sole carbon source were operated anaerobically. The changes in abundance of the cluster II Spirochaetes, two groups of acetoclastic methanogens (Methanosaetaceae and Methanosarcinaceae), and two groups of hydrogenotrophic methanogens (Methanomicrobiales and Methanobacteriales) in the reactors were assessed using qPCR targeting the 16S rRNA genes of each group. Increase in the cluster II Spirochaetes (9.0±0.4-fold) was positively correlated with increase in hydrogenotrophic methanogens, especially Methanomicrobiales (5.6±1.0-fold), but not with acetoclastic methanogens. In addition, the activity of the cluster II Spirochaetes decreased (4.6±0.1-fold) in response to high hydrogen partial pressure, but their activity was restored after consumption of hydrogen by the hydrogenotrophic methanogens. These results strongly suggest that the cluster II Spirochaetes are involved in syntrophic acetate oxidation in anaerobic digesters.

References

Jul 6, 2006·Applied and Environmental Microbiology·Dimitar KarakashevIrini Angelidaki
Jan 1, 2008·Microbes and Environments·Satoshi Hattori
Mar 20, 2012·Journal of Environmental Monitoring : JEM·Sang-Hoon LeeHee-Deung Park
Apr 9, 2013·Bioresource Technology·Sang-Hoon LeeHee-Deung Park

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Citations

Jan 20, 2017·International Journal of Systematic and Evolutionary Microbiology·Janina S KoelschbachRainer U Meckenstock
Dec 1, 2017·International Journal of Environmental Research and Public Health·Jing WuYi Qian
Sep 18, 2017·Bioresource Technology·Yamrot M AmhaAdam L Smith
Jul 22, 2021·Bioscience of Microbiota, Food and Health·Yan SunWen-Hui Lee

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