Sep 1, 1975

Evidence of tandem duplication of genes in a merodiploid region of Pneumococcal mutants resistant to sulfonamide

Genetics
S V Kashmiri, R D Hotchkiss

Abstract

A Pneumococcal mutant, sulr-c, resistant to sulfonamides, and three transformants bearing associated d or d+ resistance markers have earlier been reported to be unstable and show distinct patterns and frequencies of segregating stable progeny lacking the c marker. Each of the four strains showed a characteristic dosage of the genes involved in the merodiploidy. Complementary strands of DNA's from these stable and unstable strains were resolved and homoduplex and heteroduplex hybrids made from the separated DNA strands were used as donors in genetic transformations. Activities of a normal marker (streptomycin resistance) and those involved in the heterozygosity (c, d and d+) were quantitatively measured. From those heteroduplexes made up of opposite strands derived from a heterozygote and a stable strain, the normal marker is transferred efficiently, but the heterozygous markers are not. On the other hand, if both strands of a heteroduplex are derived from different heterozygotic strains, all markers can be transferred with usual efficiency to a stable recipient strain. The lowered efficiency in the former type of heteroduplex is attributed to an inhomology resulting from a tandem duplication in the merodiploid strains, and a po...Continue Reading

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Mentioned in this Paper

Antibiotic Resistance, Microbial
Transformation, Genetic
DNA Repair
Streptomycin
Hybrids
Sulfonamides
Tandem
Sulfonamide Anti-Infective Agents
Mutant
Sulfonamides, Gynecological

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