PMID: 20103986Jan 28, 2010Paper

Evidence of the existence of adenylyl cyclase 10 (ADCY10) ortholog proteins in the heads and connecting pieces of boar spermatozoa

The Journal of Reproduction and Development
Shunsuke TateHiroshi Harayama

Abstract

The aim of this study is to provide evidence of the existence of the adenylyl cyclase 10 (ADCY10) ortholog proteins in boar spermatozoa. Experiments with RT-PCR techniques, nucleotide sequence analyses and Northern blot analyses revealed that boar testes exclusively express approximately 5.1-kbp RNA, the nucleotide sequence of which is highly similar to that of human ADCY10. Database analyses with CDART suggested that pig ADCY10 ortholog proteins conserve two catalytic domains of adenylyl cyclase. Western blot techniques and indirect immunofluorescence with a specific antiserum to pig recombinant ADCY10 ortholog proteins showed that 48-kDa and 70-kDa truncated forms of pig ADCY10 ortholog proteins are localized in the equatorial segments and connecting pieces of boar ejaculated spermatozoa. Finally, cell imaging techniques with fluo-3/AM indicated that incubation with sodium bicarbonate (an ADCY10 activator) can initiate the calcium influx in the boar sperm heads that is controlled via the cyclic AMP signaling cascades. These results are consistent with the suggestion that functional ADCY10 ortholog proteins exist in the heads of boar spermatozoa. This is the first direct evidence of the existence of ADCY10 proteins in the head...Continue Reading

References

Mar 1, 1976·Journal of Reproduction and Fertility·R H HunterP J Henfrey
Feb 1, 1995·Molecular Reproduction and Development·P J AshworthP F Watson
Aug 1, 1993·Biology of Reproduction·S ParkkilaS Kellokumpu
Jul 23, 1998·Biology of Reproduction·P E Visconti, G S Kopf
Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·J BuckL R Levin
Mar 29, 2000·Molecular Reproduction and Development·M L SinclairL R Levin
Jun 24, 2000·Journal of Reproduction and Fertility·A M PetrounkinaE Töpfer-Petersen
Jul 13, 2000·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Z ZhangW Miller
Jun 26, 2001·The Journal of Biological Chemistry·B S Jaiswal, M Conti
May 4, 2002·Molecular and Cellular Endocrinology·Haim Breitbart
Dec 24, 2002·The Journal of Biological Chemistry·Ignacio A DemarcoPablo E Visconti
Sep 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bijay S Jaiswal, Marco Conti
Aug 27, 2004·Journal of Molecular Histology·Elisabeth EkstedtYvonne Ridderstråle
Oct 1, 2004·Molecular Reproduction and Development·Steve TardifJanice L Bailey
Mar 8, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Haim BreitbartSara Rubinstein
Oct 27, 2005·Molecular Reproduction and Development·James A ChaloupkaJochen Buck
Jan 13, 2006·The Journal of Biological Chemistry·María T BranhamClaudia N Tomes
Jul 18, 2006·Developmental Biology·Fang XieMarco Conti
Nov 2, 2006·The Journal of Reproduction and Development·Reiko AmanoHiroshi Harayama
May 26, 2007·Biochemical and Biophysical Research Communications·Carmen BeltránAlberto Darszon
Mar 22, 2008·Molecular Reproduction and Development·Masako KanetoKiyoshi Miki
Jun 24, 2009·The Journal of Biological Chemistry·María T BranhamClaudia N Tomes

❮ Previous
Next ❯

Citations

Sep 10, 2013·Fertility and Sterility·Agnieszka MalcherMaciej Kurpisz
Jan 21, 2012·Biology of Reproduction·Ying LiuLi-Ming Chen
Nov 1, 2018·Reproductive Medicine and Biology·Hiroshi Harayama

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.