Evidence of the generation of isosaccharinic acids and their subsequent degradation by local microbial consortia within hyper-alkaline contaminated soils, with relevance to intermediate level radioactive waste disposal

PloS One
Simon P RoutP Humphreys

Abstract

The contamination of surface environments with hydroxide rich wastes leads to the formation of high pH (>11.0) soil profiles. One such site is a legacy lime works at Harpur Hill, Derbyshire where soil profile indicated in-situ pH values up to pH 12. Soil and porewater profiles around the site indicated clear evidence of the presence of the α and β stereoisomers of isosaccharinic acid (ISA) resulting from the anoxic, alkaline degradation of cellulosic material. ISAs are of particular interest with regards to the disposal of cellulosic materials contained within the intermediate level waste (ILW) inventory of the United Kingdom, where they may influence radionuclide mobility via complexation events occurring within a geological disposal facility (GDF) concept. The mixing of uncontaminated soils with the alkaline leachate of the site resulted in ISA generation, where the rate of generation in-situ is likely to be dependent upon the prevailing temperature of the soil. Microbial consortia present in the uncontaminated soil were capable of surviving conditions imposed by the alkaline leachate and demonstrated the ability to utilise ISAs as a carbon source. Leachate-contaminated soil was sub-cultured in a cellulose degradation product...Continue Reading

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Citations

Jan 15, 2017·Applied and Environmental Microbiology·C J CharlesP N Humphreys
May 28, 2020·FEMS Microbiology Ecology·Naji M BassilJonathan R Lloyd
Mar 28, 2018·International Journal of Systematic and Evolutionary Microbiology·Naji M Bassil, Jonathan R Lloyd
Dec 22, 2020·Frontiers in Microbiology·Richard M WormaldPaul N Humphreys

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Methods Mentioned

BETA
electrophoresis
PCR
ISA

Software Mentioned

Basic Local Alignment Search Tool ( MegaBLAST )
Mothur
BACVis
- Multiple Alignment
MegaBLAST
MUSCLE
SPSS

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