PMID: 9658009Jul 11, 1998Paper

Evidence of two oxidative reaction steps initiating anaerobic degradation of resorcinol (1,3-dihydroxybenzene) by the denitrifying bacterium Azoarcus anaerobius

Journal of Bacteriology
B Philipp, B Schink

Abstract

The denitrifying bacterium Azoarcus anaerobius LuFRes1 grows anaerobically with resorcinol (1,3-dihydroxybenzene) as the sole source of carbon and energy. The anaerobic degradation of this compound was investigated in cell extracts. Resorcinol reductase, the key enzyme for resorcinol catabolism in fermenting bacteria, was not present in this organism. Instead, resorcinol was hydroxylated to hydroxyhydroquinone (HHQ; 1,2,4-trihydroxybenzene) with nitrate or K3Fe(CN)6 as the electron acceptor. HHQ was further oxidized with nitrate to 2-hydroxy-1,4-benzoquinone as identified by high-pressure liquid chromatography, UV/visible light spectroscopy, and mass spectroscopy. Average specific activities were 60 mU mg of protein-1 for resorcinol hydroxylation and 150 mU mg of protein-1 for HHQ dehydrogenation. Both activities were found nearly exclusively in the membrane fraction and were only barely detectable in extracts of cells grown with benzoate, indicating that both reactions were specific for resorcinol degradation. These findings suggest a new strategy of anaerobic degradation of aromatic compounds involving oxidative steps for destabilization of the aromatic ring, different from the reductive dearomatization mechanisms described s...Continue Reading

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Citations

Apr 3, 1999·FEMS Microbiology Reviews·R HilleW Reichenbecher
May 3, 2000·Journal of Applied Microbiology·A ChauhanR K Jain
Mar 5, 2009·Microbiology and Molecular Biology Reviews : MMBR·Manuel CarmonaEduardo Díaz
Jun 14, 2013·Environmental Microbiology Reports·Bodo Philipp, Bernhard Schink
Jun 22, 2002·FEMS Microbiology Letters·Bodo PhilippBernhard Schink
Nov 8, 2020·Journal of Hazardous Materials·Li-Hui YangAi-Jie Wang
Feb 5, 2013·Biochimica Et Biophysica Acta·Stéphane GrimaldiAxel Magalon
Apr 29, 2006·Journal of Natural Products·Anton PohankaAnders Broberg
May 26, 2009·Chemical Research in Toxicology·Krzysztof J ReszkaBradley E Britigan
Nov 26, 2014·Environmental Science & Technology·Elizabeth A Pillar-LittleMarcelo I Guzman

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