Evidence of viral capsid dynamics using limited proteolysis and mass spectrometry.

The Journal of Biological Chemistry
B BothnerG Siuzdak

Abstract

Virus particles are stable yet exhibit highly dynamic character given the events that shape their life cycle. Isolated from their hosts, the nucleoprotein particles are macromolecules that can be crystallized and studied by x-ray diffraction. During assembly, maturation and entry, however, they are highly dynamic and display remarkable plasticity. These dynamic properties can only be inferred from the x-ray structure and must be studied by methods that are sensitive to mobility. We have used matrix-assisted laser desorption/ionization mass spectrometry combined with time resolved, limited proteolysis (Cohen, S. L., Ferre-D'Amare, A. R., Burley, S. K., and Chait, B. T. (1995) Protein Sci. 4, 1088-1099; Kriwacki, R. W., Wu, J., Tennant, T., Wright, P. E., and Siuzdak, G. (1997) J. Chromatogr. 777, 23-30; Kriwacki, R. W., Wu, J., Siuzdak, G., and Wright, P. E. (1996) J. Am. Chem. Soc. 118, 5320-5321) to examine the viral capsid of flock house virus. Employing less than 10 microg of virus, time course digestion products were assigned to polypeptides of the subunit. Although surface regions in the three-dimensional structure were susceptible to cleavage on extended exposure to the protease, the first digestion products were invariab...Continue Reading

References

Nov 1, 1990·Biomedical & Environmental Mass Spectrometry·J J GormanB J Shiell
Jun 1, 1995·Protein Science : a Publication of the Protein Society·S L CohenB T Chait
Jan 1, 1995·Rapid Communications in Mass Spectrometry : RCM·R W NelsonP Williams
Jan 1, 1996·Chemistry & Biology·G SiuzdakC M Chang
Sep 1, 1996·Genetic Analysis : Biomolecular Engineering·C JurinkeH Köster

❮ Previous
Next ❯

Citations

Nov 26, 2002·Journal of Molecular Recognition : JMR·Marc H V Van Regenmortel
Feb 9, 2002·Mass Spectrometry Reviews·C Fenselau, P A Demirev
Sep 5, 2002·Mass Spectrometry Reviews·Igor A Kaltashov, Stephen J Eyles
Jan 18, 2005·Journal of the American Society for Mass Spectrometry·Justin G StrohJulie Xie
May 25, 2007·Journal of the American Society for Mass Spectrometry·Moo-Jin SuhPatrick A Limbach
Apr 11, 2008·Journal of the American Society for Mass Spectrometry·Li TaoMichael L Doyle
Oct 22, 2003·Current Opinion in Structural Biology·Kelly K Lee, John E Johnson
Nov 20, 2003·Trends in Biotechnology·Adam Zlotnick, Stephen J Stray
Jun 11, 2002·Journal of Molecular Biology·Thomas J SmithCharles A Stanley
Apr 8, 2000·Current Opinion in Structural Biology·J E Johnson, W Chiu
Apr 20, 2013·Journal of the American Chemical Society·Jessica Z BereszczakAlbert J R Heck
Dec 27, 2012·ACS Nano·Leonard H Rome, Valerie A Kickhoefer
Feb 12, 2008·Nature Structural & Molecular Biology·Shee-Mei LokMichael G Rossmann
Jan 8, 2013·Microscopy·John E Johnson
May 10, 2001·Protein Science : a Publication of the Protein Society·L M IakouchevaE J Ackerman
Apr 13, 2007·Journal of Virology·Umesh Katpally, Thomas J Smith
Jun 3, 2011·Journal of Virology·Reza KhayatJohn E Johnson
Oct 19, 2012·Journal of Virology·Yang YangFelipe Diaz-Griffero
Apr 17, 2009·Journal of Virology·Manidipa BanerjeeJohn E Johnson
Mar 9, 2012·Journal of Virology·Jun LinDavid M Belnap
Dec 14, 2011·Journal of Virology·Vincent C LucaDaved H Fremont
May 14, 2008·Annual Review of Phytopathology·Mark YoungTrevor Douglas
Dec 12, 2012·PloS One·Shee-Mei LokScott F Michael
May 5, 2004·Proceedings of the National Academy of Sciences of the United States of America·George RiceMark Young
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·J K LewisG Siuzdak
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·K BrooG Siuzdak
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·J K LewisG Siuzdak
Nov 3, 2014·Annual Review of Virology·Theodore C Pierson, Michael S Diamond
Sep 27, 2013·Journal of Virology·Vamseedhar RayaproluBrian Bothner
Mar 25, 2009·Cellular and Molecular Life Sciences : CMLS·Kaare TeilumBirthe B Kragelund
Feb 17, 2001·Journal of Molecular Biology·R TumaP E Prevelige
Jul 1, 2008·Antiviral Research·Rushika PereraRichard J Kuhn
May 24, 2008·Mass Spectrometry Reviews·Victoria L MortonAlison E Ashcroft

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.