Evidence supporting a two-receptor model for insulin binding by cultured embryonic heart cells

Endocrinology
F B WheelerL J Elsas

Abstract

Curvilinear Scatchard plots for insulin binding by cultured embryonic heart cells at 15 C were interpreted assuming a two-receptor model (Santora II, A. C., F.B. Wheeler, R.L. DeHaan, and L.J. Elsas II, Endocrinology 104:1059, 1979). The present studies test this hypothesis further. Both 125I-labeled hormone binding and 2-methylamino-[1-14C]isobutyric acid ([14C]meAIB) transport were assayed at 24 C in identical buffers. Equilibrium chicken insulin-binding experiments yielded curvilinear Scatchard functions, consistent with binding to both high affinity, low capacity and low affinity, high capacity receptor sites at 24 C. Insulin-stimulated transport of the model amino acid [14C]meAIB (a specific alanine-preferring system substrate) paralleled the expected occupancy of the low affinity receptors. In competitive binding assays, the abilities of multiplication-stimulating activity (MSA), proinsulin, and insulin to compete for primarily high affinity insulin binding were compared with their abilities to stimulate transport. The relative potencies for binding competition with 34 pM chicken [125I]insulin were insulin greater than proinsulin greater than or equal to MSA (1:0,05:0.03). In contrast, the relative potencies for stimulati...Continue Reading

Citations

Jun 1, 1981·Developmental Biology·J C SerravezzaL J Elsas
Nov 1, 1985·Molecular and Cellular Endocrinology·D Turyn, J M Dellacha
May 24, 1983·Biochimica Et Biophysica Acta·M A ShotwellD L Oxender
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·C C WangP De Meyts
Sep 1, 1984·Scandinavian Journal of Clinical and Laboratory Investigation·K PetterssonE Kokko
Jan 1, 1981·Journal of Supramolecular Structure and Cellular Biochemistry·M M RechlerR M White
Mar 1, 1984·Clinics in Endocrinology and Metabolism
Mar 1, 1984·The American Journal of Physiology·R L DeHaanL J Elsas
Jul 1, 1989·Metabolism: Clinical and Experimental·N LongoL J Elsas
Mar 1, 1984·Clinics in Endocrinology and Metabolism·S P Nissley, M M Rechler

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