Evidence that a viral replicase protein is involved in the disassembly of tobacco mosaic virus particles in vivo

Virology
X Wu, J G Shaw

Abstract

Tobacco mosaic virus (TMV) particles have been shown to undergo bidirectional disassembly when they are introduced into host cells. Approximately three-quarters of the genomic RNA (i.e., the 126-kDa and 183-kDa protein ORFs) is first uncoated in the 5'-to-3' direction and the process is then completed by removal of coat protein molecules in the 3'-to-5' direction. An effort was made to determine whether the 126-kDa protein or the 183-kDa protein, both of which are involved in replication of the viral RNA, is required for the second part of the disassembly reaction. It was shown that progeny negative-strand viral RNA begins to be produced in inoculated cells at about the same time that 3'-to-5' disassembly is initiated thus suggesting that the two processes may be coupled. Particles containing mutant forms of the viral RNA in which large sections of the 126-kDa and 183-kDa protein ORFs were missing were not disassembled in the 3'-to-5' direction when they were introduced into cells. However, they were disassembled when the inoculum contained purified TMV RNA from which, presumably, the two functional proteins could be translated Particles containing mutants of the RNA from which a few codons had been deleted in or near conserved...Continue Reading

References

Jun 1, 1985·The Journal of General Virology·T M Wilson
May 5, 1988·Nature·A E GorbalenyaV M Blinov
Nov 11, 1986·Nucleic Acids Research·M IshikawaY Okada
Feb 1, 1984·The Journal of General Virology·P J Butler
Apr 1, 1994·Virology·X WuJ G Shaw
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·X Wu, J Shaw
Aug 1, 1957·Virology·H FRAENKEL-CONRAT

❮ Previous
Next ❯

Citations

May 1, 2001·Molecular Plant Pathology·E Knapp, D J Lewandowski
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J G Shaw
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·K W Buck
Jul 18, 2000·Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme·Y Okada
Jul 31, 2002·Annual Review of Phytopathology·James N Culver
Jun 7, 2014·Annual Review of Phytopathology·Pooja Saxena, George P Lomonossoff
Mar 12, 2015·Virology·Laura R Newburn, K Andrew White
Jul 27, 2002·Annual Review of Microbiology·Minna M PoranenDennis H Bamford

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.