Evidence that assembly of a potyvirus begins near the 5' terminus of the viral RNA

The Journal of General Virology
X Wu, J G Shaw

Abstract

A search for the first region in the genomic RNA of a potyvirus to be encapsidated during the assembly of virus particles in vivo has been undertaken. Protoplasts were collected at various times after inoculation and fragments of viral RNA that were protected from nuclease degradation were isolated from extracts and identified by RT-PCR procedures. Nuclease-resistant fragments of viral RNA were not detected in protoplasts that had been infected for 30 min. However, such fragments were present in protoplasts collected 40 min after inoculation and these contained a region at or near the 5' terminus of the viral RNA. Protoplasts that had been infected for 45 min or longer contained full-length viral RNA in a nuclease-resistant form. These results suggest that assembly of virus particles begins with the interaction of coat protein subunits with the 5' terminal region of progeny viral RNA molecules.

Citations

Mar 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Valera V PeremyslovValerian V Dolja
Jan 30, 2015·Journal of Virology·Jane Besong-NdikaKristiina Mäkinen
Aug 15, 2003·The Journal of General Virology·Odile HemmerChristiane Fritsch
Feb 16, 2018·Journal of Virology·Araiz GalloJuan Antonio García
Jul 23, 2019·Science Advances·Andreja KežarMarjetka Podobnik

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