Evidence that bacteria prefer to adhere to thrombus

ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs
H M BosS F Mohammad

Abstract

This study was undertaken to investigate the possible association between thrombosis and infection using an in vitro test model in which fresh bovine blood was recirculated through test conduits (3.5 mm inner diameter) containing stent-like devices. Anticoagulation was adjusted so that the recirculating blood deposited thrombi on the stent to cause gradual occlusion, thus impeding the flow. Four stent-like devices were placed in separate conduits in each experiment, and blood was recirculated with the help of pneumatically driven ventricles. Flow through these conduits was monitored by ultrasonic flow detection. To quantitate bacterial interaction with thrombi, Staphylococcus epidermidis (15E10(9)) was labeled with 111Indium-oxine and added to the blood. Experiments lasted until the flow in the test conduits dropped to 10% of the starting flow. During this recirculation, as flow gradually decreased, one stent was taken out when flow was still at 100%, the second at 75%, the third at 50%, and the fourth at 10% of the starting flow. The number of bacteria associated with the thrombus was measured by gamma counting. The following observations were made: 1) the amount of thrombus increased with time in all experiments (this was con...Continue Reading

Citations

Feb 5, 2000·Cardiovascular and Interventional Radiology·A M MalekC F Dowd
May 26, 2001·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·P DidisheimM S Shive

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