PMID: 9182814Jun 1, 1997Paper

Evidence that beta1 integrins in keratinocyte cell-cell junctions are not in the ligand-occupied conformation

The Journal of Investigative Dermatology
L T Kim, K M Yamada

Abstract

Integrins are a family of heterodimeric cell surface molecules that function as adhesion receptors in cell-cell and cell-extracellular matrix contact. Integrins of the beta1 family are found on keratinocytes clustered at sites of cell-cell junctions both in culture and in normal skin. The possibility that these integrins function in cell-cell adhesion has been both supported and refuted in recent conflicting publications. Rather than testing further for the presence or absence of an interaction, we present evidence to show that beta1 integrins in keratinocyte cell-cell junctions are in the non-ligand-occupied conformation. We transfected keratinocytes with a construct that expresses a chimeric cell surface molecule containing the integrin beta1 cytoplasmic tail. This chimera is thought to mimic the ligand-occupied receptor and has previously been shown to be actively localized to focal adhesions in fibroblasts. We find that keratinocytes are also able to localize this chimera in focal adhesions but do not localize it to areas of cell-cell junctions. A monoclonal anti-beta1 antibody that has been previously shown to preferentially recognize ligand-occupied beta1 receptors was used to stain keratinocytes. This antibody showed sta...Continue Reading

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Citations

Nov 11, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Joseph A Depasquale
Apr 30, 2002·Cell Biochemistry and Function·Ronan O'LearyEdward J Wood
Jun 15, 2011·Annual Review of Cell and Developmental Biology·Johanna Ivaska, Jyrki Heino
May 19, 2000·The Journal of Surgical Research·L T KimR H Turnage
Feb 13, 2001·Experimental Cell Research·J D Whittard, S K Akiyama
Dec 6, 1997·Current Opinion in Genetics & Development·E FuchsQ C Yu
Jul 17, 1998·The Journal of Investigative Dermatology·P F PeñasA García-Díez

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