Evidence that bilayer bending rigidity affects membrane protein folding

Biochemistry
Paula J BoothP Wright

Abstract

The regeneration kinetics of the integral membrane protein bacteriorhodopsin have been investigated in a lipid-based refolding system. Previous studies on bacteriorhodopsin regeneration have involved detergent-based systems, and in particular mixed dimyristoylphosphatidylcholine (DMPC)/CHAPS micelles. Here, we show that the short chain lipid dihexanoylphosphatidylcholine (DHPC) can be substituted for the detergent CHAPS and that bacteriorhodopsin can be regenerated to high yield in mixed DMPC/DHPC micelles. Bacteriorhodopsin refolding kinetics are measured in the mixed DMPC/DHPC micelles. Rapid, stopped flow mixing is employed to initiate refolding of denatured bacterioopsin in SDS micelles with mixed DMPC/DHPC micelles and time-resolved fluorescence spectroscopy to follow changes in protein fluorescence during folding. Essentially identical refolding kinetics are observed for mixed DMPC/CHAPS and mixed DMPC/DHPC micelles. Only one second-order retinal/apoprotein reaction is identified, in which retinal binds to a partially folded apoprotein intermediate, and the free energy of this retinal binding reaction is found to be the same in both types of mixed micelles. Formation of the partially folded apoprotein intermediate is a ra...Continue Reading

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Citations

Jun 11, 2002·Journal of Molecular Biology·Wim Meijberg, Paula J Booth
Aug 17, 1999·Current Opinion in Structural Biology·S K Buchanan
Sep 25, 2003·Bioorganic & Medicinal Chemistry Letters·Hirotaka SasakiKazuo Tachibana
Jan 17, 2012·Biochemistry·Venkatramanan KrishnamaniJanos K Lanyi
Jan 8, 2013·Lab on a Chip·Dirk van Swaay, Andrew deMello
Jan 15, 2009·Proceedings of the National Academy of Sciences of the United States of America·Paul Curnow, Paula J Booth
Oct 28, 2010·Protein Engineering, Design & Selection : PEDS·Daniel E Otzen
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·S H White, W C Wimley
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·U HauptsD Oesterhelt
May 3, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Weihua YeLena Mäler
Apr 6, 2012·Current Opinion in Structural Biology·Paula J Booth
Apr 7, 2010·Journal of Biotechnology·Oksana V NekrasovaMikhail P Kirpichnikov
Aug 28, 2012·Chemical Reviews·Ulrich H N DürrAyyalusamy Ramamoorthy
Nov 5, 2014·Journal of the American Chemical Society·Jonathan P SchlebachChiwook Park
Dec 15, 2015·Magnetic Resonance in Chemistry : MRC·Jobst LiebauLena Mäler
Nov 13, 2007·Journal of Molecular Biology·Craig McKibbinPaula J Booth
Apr 25, 2008·Protein Science : a Publication of the Protein Society·Damien Thévenin, Tzvetana Lazarova
Sep 8, 2004·Journal of Molecular Biology·Samantha J AllenPaula J Booth
Sep 8, 2004·Journal of Molecular Biology·Samantha J AllenPaula J Booth
Nov 3, 2004·Biochimica Et Biophysica Acta·Annela M SeddonPaula J Booth
Nov 10, 2004·Biochimica Et Biophysica Acta·Iren Constantinescu, Michel Lafleur
Feb 27, 1999·Current Opinion in Structural Biology·P J Booth, A R Curran
Jan 24, 2006·Journal of Molecular Biology·Emma L R ComptonPaula J Booth
Feb 9, 2006·Systematic and Applied Microbiology·Clarissa Schwab, Michael G Gänzle
Oct 6, 2000·Biochimica Et Biophysica Acta·S C CostiganR H Templer
Jul 25, 2014·Archives of Biochemistry and Biophysics·Jennifer NeumannDirk Schneider
Mar 24, 2015·Chemical Communications : Chem Comm·Yuval ElaniOscar Ces
Jun 21, 2008·Journal of Molecular Biology·Annela M SeddonPaula J Booth
Mar 16, 2011·Biophysical Journal·Venkatramanan Krishnamani, Janos K Lanyi
Mar 3, 2004·Biophysical Journal·Dirk LinkeVictor I Emelyanenko
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Aug 12, 2016·The Journal of Physical Chemistry Letters·Erin E SchulerR Brian Dyer
Nov 2, 1999·Journal of Molecular Biology·L E FisherJ N Sturgis

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