Evidence That Calcium Entry Into Calcium-Transporting Dental Enamel Cells Is Regulated by Cholecystokinin, Acetylcholine and ATP

Frontiers in Physiology
M K NurbaevaR S Lacruz

Abstract

Dental enamel is formed by specialized epithelial cells which handle large quantities of Ca2+ while producing the most highly mineralized tissue. However, the mechanisms used by enamel cells to handle bulk Ca2+ safely remain unclear. Our previous work contradicted the dogma that Ca2+ is ferried through the cytosol of Ca2+-transporting cells and instead suggested an organelle-based route across enamel cells. This new paradigm involves endoplasmic reticulum (ER)-associated Ca2+ stores and their concomitant refilling by store-operated Ca2+ entry (SOCE) mediated by Ca2+ release activated Ca2+ (CRAC) channels. Given that Ca2+ handling is maximal during the enamel-mineralization stage (maturation), we anticipated that SOCE would also be elevated then. Confirmation was obtained here using single-cell recordings of cytosolic Ca2+ concentration ([Ca2+]cyt) in rat ameloblasts. A candidate SOCE agonist, cholecystokinin (CCK), was found to be upregulated during maturation, with Cck transcript abundance reaching 30% of that in brain. CCK-receptor transcripts were also detected and Ca2+ imaging showed that CCK stimulation increased [Ca2+]cyt in a dose-responsive manner that was sensitive to CRAC-channel inhibitors. Similar effects were obser...Continue Reading

References

Feb 1, 1992·The American Journal of Physiology·J J FeherR H Wasserman
Jun 1, 1993·Pharmacology & Therapeutics·M P Caulfield
Jan 1, 1997·Annual Review of Physiology·R A Liddle
May 29, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·C E Smith
Jan 2, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·M J Hubbard
Oct 21, 2004·The Journal of Biological Chemistry·Chris I TurnbullMichael J Hubbard
Mar 25, 2005·Physiological Reviews·Anant B Parekh, James W Putney
May 9, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jens F Rehfeld
Apr 13, 2007·Physiological Reviews·J Kevin FoskettDon-On Daniel Mak
Apr 18, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jaime CarrilloJavier Alonso
Jun 23, 2007·Current Topics in Medicinal Chemistry·Jens F RehfeldThomas V O Hansen
Aug 19, 2007·Journal of Neurochemistry·Katsuhiko Mikoshiba
Dec 28, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Chun-Min LoPatrick Tso
Oct 30, 2008·American Journal of Physiology. Endocrinology and Metabolism·Ixchel M BrennanChristine Feinle-Bisset
Dec 17, 2009·The Journal of Allergy and Clinical Immunology·Christie-Ann McCarlStefan Feske
Aug 24, 2010·Molecular Interventions·James W Putney
Jan 25, 2012·European Journal of Oral Sciences·Michael J HubbardJonathan E Mangum
Jun 9, 2012·Cells, Tissues, Organs·Ping HuMichael L Paine
Dec 12, 2012·Cell Calcium·Isabella DerlerChristoph Romanin
Dec 18, 2012·Journal of Dental Research·R S LacruzM L Paine
Jul 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Julia V GerasimenkoOle H Petersen
Mar 14, 2014·Journal of Dental Research·S WangJ C Hu
Aug 2, 2015·Journal of Dental Research·M K NurbaevaR S Lacruz
Sep 25, 2015·Physiological Reviews·Murali Prakriya, Richard S Lewis
Oct 31, 2015·Scientific Reports·Meerim K NurbaevaRodrigo S Lacruz
Mar 30, 2017·JCI Insight·Miriam EcksteinRodrigo S Lacruz

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Citations

Feb 20, 2020·Science Signaling·Francisco J AulestiaRodrigo S Lacruz
Jun 16, 2019·Cell Calcium·Lisa J RobinsonJonathan Soboloff
May 1, 2021·International Journal of Molecular Sciences·Kristóf KádárÁkos Zsembery
Aug 31, 2021·Frontiers in Endocrinology·Veronica CostinitiRodrigo S Lacruz
Jan 28, 2022·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Veronica CostinitiRodrigo S Lacruz

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