Abstract
Phe-pro-arg-chloromethyl ketone-inhibited alpha-thrombin [FPR alpha-thr] retains its fibrinogen recognition site (exosite 1), augments fibrin/fibrinogen [fibrin(ogen)] polymerization, and increases the incorporation of fibrin into clots. There are two 'low-affinity' thrombin-binding sites in each central E domain of fibrin, plus a non-substrate 'high affinity' gamma' chain thrombin-binding site on heterodimeric 'fibrin(ogen) 2' molecules (gamma(A), gamma'). 'Fibrin(ogen) 1' (gamma(A), gamma(A)) containing only low-affinity thrombin-binding sites, showed concentration-dependent FPR alpha-thr enhancement of polymerization, thus indicating that low-affinity sites are sufficient for enhancing polymerization. FPR gamma-thr, whose exosite 1 is non-functional, did not enhance polymerization of either fibrin(ogen)s 1 or 2 and DNA aptamer HD-1, which binds specifically to exosite 1, blocked FPR alpha-thr enhanced polymerization of both types of fibrin(ogen) (1>2). These results showed that exosite 1 is the critical element in thrombin that mediates enhanced fibrin polymerization. Des B beta 1-42 fibrin(ogen) 1, containing defective 'low-affinity' binding sites, was subdued in its FPR alpha-thr-mediated reactivity, whereas des B beta 1-4...Continue Reading
References
Apr 1, 1992·Protein Science : a Publication of the Protein Society·W BodeA Karshikov
Nov 24, 1992·Biochemistry·K P Hopfner, E Di Cera
Feb 6, 1992·Nature·L C BockJ J Toole
Jan 8, 1991·Biochemistry·B V PandyaA Z Budzynski
Sep 6, 1988·Biochemistry·J W FentonG D Wilner
Mar 15, 1987·The Biochemical Journal·M Kaminski, J McDonagh
Nov 19, 1985·Biochemistry·L J BerlinerJ W Fenton
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·C Wolfenstein-Todel, M W Mosesson
Jul 14, 1995·The Journal of Biological Chemistry·M TsiangC S Gibbs
Jan 1, 1993·Thrombosis Research·M T Stubbs, W Bode
Aug 13, 1996·Biochemistry·K R SiebenlistM W Mosesson
Sep 20, 1996·The Journal of Biological Chemistry·D A MehM W Mosesson
Jun 27, 2000·Biophysical Journal·S BernoccoM Rocco
Feb 15, 2001·The Biochemical Journal·K HosokawaT Koide
Jul 4, 2001·Thrombosis Research·D A MehG Di Minno
Jul 20, 2001·Annals of the New York Academy of Sciences·M RoccoF Ferri
Mar 29, 2003·Blood·Amy V CooperRobert A S Ariëns
Apr 19, 2003·The Journal of Biological Chemistry·Caroline H PospisilJeffrey I Weitz
Jul 12, 2003·Science·Reha CelikelZaverio M Ruggeri
Jul 12, 2003·Science·John J DumasLidia Mosyak
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·R S LovelyD H Farrell
Feb 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Igor PechikLeonid Medved
Citations
Sep 14, 2011·Circulation·Arne LauerChristian Foerch
Jul 9, 2005·Blood·Kevin R SiebenlistLaurie Stojanovic
Aug 23, 2012·PloS One·Ferdinand BohmannWaltraud Pfeilschifter
Jun 12, 2009·European Biophysics Journal : EBJ·M MissoriM De Spirito
Sep 24, 1998·Biophysical Journal·E Di StasioE Di Cera
Aug 8, 2013·Stroke; a Journal of Cerebral Circulation·Sae-Yeon WonRajiv Gupta