Evidence that cell surface charge reduction modifes capillary red cell velocity-flux relationships in hamster cremaster muscle

The Journal of Physiology
Hans VinkJos A E Spaan

Abstract

1. From capillary red cell velocity (V)-flux (F) relationships of hamster cremaster muscle a yield velocity (VF = 0) can be derived at which red cell flux is zero. Red cell velocity becomes intermittent and/or red blood cells come to a complete standstill for velocities close to this yield velocity, and, at the same time, capillary tube haematocrit becomes very low. 2. We have tested whether the net negative charge of red blood cells (RBCs) contributes to the magnitude of VF = 0. Velocity-flux relationships were measured for normal cells, normal cells labelled with the fluorescent dye calcein (LRBCs), and red cells treated with hexadimethrine to mask negative charge and labelled with calcein as well (HDM-LRBCs). Measurements were done in a hamster cremaster muscle preparation applying video in vivo microscopy. 3. Hexadimethrine treatment reduced the net negative surface charge of red cells to 20% of control as estimated from transmission electron microscopy using a ferritin tagging technique. The values of VF = 0 found for normal red cells and HDM-LRBCs were 86 +/- 15 and 31 +/- 17 microns s-1, +/- S.E.M., n = 12, respectively, which were significantly different (P < 0.05). For normal cells and cells labelled with calcein only,...Continue Reading

Citations

Apr 20, 2014·Basic Research in Cardiology·Leif OstergaardHans Erik Bøtker
Jan 18, 2013·Transfusion·Kevin R Ward
Jul 17, 2014·Pflügers Archiv : European journal of physiology·Hans Oberleithner
Jul 5, 2012·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Gaétan ChevalierRichard M Delany
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