Evidence That Ceriporiopsis subvermispora Degrades Nonphenolic Lignin Structures by a One-Electron-Oxidation Mechanism.

Applied and Environmental Microbiology
E SrebotnikK E Hammel

Abstract

The white-rot fungus Ceriporiopsis subvermispora is able to degrade nonphenolic lignin structures but appears to lack lignin peroxidase (LiP), which is generally thought to be responsible for these reactions. It is well established that LiP-producing fungi such as Phanerochaete chrysosporium degrade nonphenolic lignin via one-electron oxidation of its aromatic moieties, but little is known about ligninolytic mechanisms in apparent nonproducers of LiP such as C. subvermispora. To address this question, C. subvermispora and P. chrysosporium were grown on cellulose blocks and given two high-molecular-weight, polyethylene glycol-linked model compounds that represent the major nonphenolic arylglycerol-(beta)-aryl ether structure of lignin. The model compounds were designed so that their cleavage via one-electron oxidation would leave diagnostic fragments attached to the polyethylene glycol. One model compound was labeled with (sup13)C at C(inf(alpha)) of its propyl side chain and carried ring alkoxyl substituents that favor C(inf(alpha))-C(inf(beta)) cleavage after one-electron oxidation. The other model compound was labeled with (sup13)C at C(inf(beta)) of its propyl side chain and carried ring alkoxyl substituents that favor C(inf...Continue Reading

References

Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·T K KirkJ G Zeikus
Jan 1, 1987·Annual Review of Microbiology·T K Kirk, R L Farrell
Sep 1, 1995·Applied and Environmental Microbiology·S KawaiK E Hammel
Dec 1, 1993·Applied and Environmental Microbiology·A B OrthM Tien
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·E SrebotnikK E Hammel
Jun 1, 1993·Applied and Environmental Microbiology·C Rüttimann-JohnsonT K Kirk

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Citations

Nov 24, 1999·FEBS Letters·A N KapichK E Hammel
Mar 23, 2012·The Journal of Biological Chemistry·Elena Fernández-FueyoAngel T Martínez
Sep 28, 2014·Applied and Environmental Microbiology·Daniel J YelleKenneth E Hammel
Jul 9, 2004·Applied and Environmental Microbiology·Anderson GuerraJohn Ralph

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