Evidence that dimers remaining in preinduced Escherichia coli B/r Hcr+ become insensitive after DNA replication to the extract from Micrococcus luteus

Biophysical Journal
M SedliakováK Kleibl

Abstract

In Escherichia coli B/r Her+ irradiated with two separate fluences, dimer excision is prematurely interrupted. The present study was designed to follow tha fate of dimers remaining unexcised. The results imply that these dimers (or distortions containing dimers) are transformed on replication from the state of sensitivity to the state of insensitivity to endonuclease from Micrococcus luteus. This conclusion is based on the following findings: (a) dimers were radiochromatographically detectable in DNA replicated after UV, which indicated that they were tolerated on replication. (b) Similar amounts of dimers were detected radiochromatographically both in DNA remaining unreplicated and DNA twice replicated after UV, This along with the low transfer of parental label into daughter DNA, indicated that dimers remained in situ in parental chains. (c) Immediately after UV, all parental DNA contained numerous sites sensitive to the extract from M. luteus. 2 h after UV, a portion of parental DNA still contained a number of endonuclease-sensitive (Es) sites, while another portion of parental DNA and all daughter DNA were free of Es sites. (d) The occurrence of parental DNA free of Es sites was not temporally correlated with dimer excision...Continue Reading

Citations

Nov 16, 1992·Mutation Research·V SlezárikováA G Skavronskaya
Sep 1, 1991·Journal of Photochemistry and Photobiology. B, Biology·V Slezáriková, M Sedliaková
May 1, 1993·Journal of Photochemistry and Photobiology. B, Biology·I FridrichováM Sedliaková
Feb 3, 1998·Journal of Photochemistry and Photobiology. B, Biology·M SedliakováM Pirsel
Dec 30, 1998·Journal of Photochemistry and Photobiology. B, Biology·M Sedliakova
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·Z Livneh
Jan 15, 1987·European Journal of Biochemistry·G BanfalviF Antoni
Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·Z LivnehT Elizur
Apr 1, 1984·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·M PirselM Sedliaková

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