PMID: 3320955Dec 10, 1987Paper

Evidence that DNA involved in the expression of nodulation (nod) genes in Rhizobium binds to the product of the regulatory gene nodD

Nucleic Acids Research
G F HongA W Johnston

Abstract

In Rhizobium leguminosarum biovar viciae, the regulatory nodulation nodD gene has at least two functions. It constitutively represses its own transcription and in the presence of inducer flavonoid molecules, it activates the expression of two other nod gene transcriptional units, nodABCIJ and nodFE. Upstream of nodA and nodF is a conserved sequence, the nod box, which has been implicated in nodD-mediated transcriptional activation of these genes. DNA fragments spanning the nod boxes that precede nodA and nodF were end-labelled and were exposed to cell-free extracts obtained from strains of Rhizobium. Using the gel retardation technique, it was shown that a complex between protein and these DNA fragments was formed, but only if the extract contained a functional nodD gene. Evidence that the protein that binds to the regulatory sequences is the nodD gene product came from the observation that a complex was formed between the nod box preceding nodA and protein from a cell-free extract isolated from Escherichia coli containing the cloned nodD gene. Extracts from Rhizobium strains containing mutant forms of nodD which were specifically affected in autoregulation or in flavonoid-dependent activation formed either no protein DNA compl...Continue Reading

References

Oct 5, 1986·Journal of Molecular Biology·M GöttfertA Kondorosi
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J T Mulligan, S R Long
Sep 1, 1974·Journal of General Microbiology·J E Beringer
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·N L LeeR G Wallace
Dec 21, 1984·Nucleic Acids Research·I TörökA Kondorosi

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Citations

Jan 1, 1989·Plant Molecular Biology·H P SpainkB E Lugtenberg
Sep 1, 1979·Molecular & General Genetics : MGG·J Ostrowski, D Hulanicka
Aug 30, 2008·Science in China. Series C, Life Sciences·H XiaoJ Zhu
Aug 30, 2008·Science in China. Series C, Life Sciences·S LiuG Hong
Jan 1, 1993·FEMS Microbiology Reviews·M Göttfert
Jan 1, 1989·Biotechnology Advances·R J Watson
Jan 1, 1993·Biotechnology Advances·P K SharmaR C Dogra
Sep 4, 1999·FEMS Microbiology Letters·L SuominenK Lindström
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·K GoethalsM Holsters
Jan 7, 2003·Applied and Environmental Microbiology·John Loh, Gary Stacey
Jul 21, 2006·Journal of Bacteriology·Melicent C PeckSharon R Long
Dec 26, 2001·Journal of Bacteriology·Kuo-Chen YehSharon R Long
Aug 17, 2000·Biochemical and Biophysical Research Communications·Y YangG Hong
Jan 1, 1996·Critical Reviews in Biotechnology·S G Pueppke
Nov 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Z GyörgypalA Kondorosi
Jun 25, 1992·Nature·R F Fisher, S R Long
Aug 7, 1999·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·S M Kuo, P S Leavitt
Aug 1, 1998·The Journal of Biological Chemistry·S T LiuG F Hong
Aug 3, 2017·Genetics and Molecular Biology·Luciane M P Passaglia
Jul 12, 2020·Applied and Environmental Microbiology·Shaun FergusonClive W Ronson
Mar 1, 1995·Microbiological Reviews·P van Rhijn, J Vanderleyden
Jun 1, 1989·Journal of Bacteriology·N Deshmane, G Stacey
Sep 1, 1989·Journal of Bacteriology·H R SchlamanB J Lugtenberg
Aug 1, 1992·Journal of Bacteriology·H R SchlamanB J Lugtenberg

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