Evidence That Dynorphin Acts Upon KNDy and GnRH Neurons During GnRH Pulse Termination in the Ewe

Endocrinology
Peyton W WeemsM N Lehman

Abstract

A subpopulation of neurons located within the arcuate nucleus, colocalizing kisspeptin, neurokinin B, and dynorphin (Dyn; termed KNDy neurons), represents key mediators of pulsatile GnRH secretion. The KNDy model of GnRH pulse generation proposes that Dyn terminates each pulse. However, it is unknown where and when during a pulse that Dyn is released to inhibit GnRH secretion. Dyn acts via the κ opioid receptor (KOR), and KOR is present in KNDy and GnRH neurons in sheep. KOR, similar to other G protein-coupled receptors, are internalized after exposure to ligand, and thus internalization can be used as a marker of endogenous Dyn release. Thus, we hypothesized that KOR will be internalized at pulse termination in both KNDy and GnRH neurons. To test this hypothesis, GnRH pulses were induced in gonad-intact anestrous ewes by injection of neurokinin B (NKB) into the third ventricle and animals were euthanized at times of either pulse onset or termination. NKB injections produced increased internalization of KOR within KNDy neurons during both pulse onset and termination. In contrast, KOR internalization into GnRH neurons was seen only during pulse termination, and only in GnRH neurons within the mediobasal hypothalamus (MBH). Overa...Continue Reading

References

Jun 1, 1978·Biology of Reproduction·G L JacksonA Zaleski
Sep 1, 1990·Endocrinology·S M MoenterF J Karsch
Jan 1, 1982·Neuroendocrinology·M KawakamiR Hayashi
Aug 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Nicolas de RouxEdwin Milgrom
Oct 24, 2003·The New England Journal of Medicine·Stephanie B SeminaraWilliam H Colledge
Sep 4, 2004·Journal of Neuroendocrinology·C H Brown
Nov 24, 2005·The Journal of Comparative Neurology·Clifford B Saper
Aug 15, 2009·Journal of Neuroendocrinology·S OhkuraH Okamura
Sep 25, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Victor M NavarroRobert A Steiner
Oct 6, 2016·Endocrinology·Chrysanthi FerganiRobert L Goodman
Apr 28, 2017·International Journal of Molecular Sciences·Kamila SkieterskaKathleen Van Craenenbroeck
Mar 17, 2018·Vitamins and Hormones·Peyton W WeemsRobert L Goodman

❮ Previous
Next ❯

Citations

Jun 9, 2018·Reproduction : the Official Journal of the Society for the Study of Fertility·Casey C NestorRobert L Goodman
Jan 23, 2019·Endocrinology·Teresa K Woodruff
Nov 8, 2018·The Journal of Endocrinology·Christopher J ScottBriony M McGrath
Jul 17, 2018·Endocrinology·Aleisha M MooreMichael N Lehman
May 28, 2019·The Journal of Clinical Endocrinology and Metabolism·Margaret F LippincottStephanie Beth Seminara
Jun 6, 2019·Frontiers in Endocrinology·Yoshihisa UenoyamaHiroko Tsukamura
Jun 24, 2020·Peptides·Richard J Bodnar
Jun 12, 2021·Molecular and Cellular Endocrinology·Mariela Faykoo-MartinezMelissa M Holmes
Jan 28, 2022·Journal of Neuroendocrinology·Christopher R McCartneySuzanne M Moenter
Feb 3, 2022·Journal of Neuroendocrinology·Robert L GoodmanVictor M Navarro

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.