PMID: 9526611Apr 4, 1998Paper

Evidence that folliculo-stellate cells mediate the inhibitory effect of Japanese kampo medicine, unkei-to, on growth hormone secretion in rat anterior pituitary cell cultures

American Journal of Reproductive Immunology : AJRI
K KoikeM Inoue

Abstract

Cytokine-induced neutrophil chemoattractant (CINC) was reported to influence anterior pituitary hormone release. We recently found that Unkei-to, one of the Japanese Kampo medicines, stimulated CINC secretion by rat anterior pituitary cells and the pituitary folliculo-stellate (FS)-like cell line (TtT/GF). Therefore, the effect of Unkei-to on growth hormone (GH) secretion by rat anterior pituitary cells was investigated. Dispersed normal anterior pituitary cells, the folliculo-stellate-like cell line TtT/GF, and the GH3 cell were used to test the effect of Unkei-to on GH secretion. In reconstitutive coculture experiments, TtT/GF cells were mixed with GH3 cells at a ratio (TtT/GF cells: GH3 cells) of 1:99. From this mixture, cells were seeded onto plates at a density of 10(4) cells/well and were cultured for 5 days. The cells were then used in the experiments. Unkei-to at 20 micrograms/ml significantly inhibited GH secretion by normal anterior pituitary cells within 12 hr of incubation. In contrast Unkei-to stimulated GH secretion by GH3 cells in a time- and dose-dependent manner, suggesting that an accessory cell type was involved. To assess the contribution of CINC as a paracrine factor, an experiment using a reconstitutive co...Continue Reading

References

Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·N FerraraD Gospodarowicz
Jul 1, 1989·Endocrinology·B L SpangeloR M MacLeod
Nov 30, 1989·Biochemical and Biophysical Research Communications·D Gospodarowicz, K Lau
Jan 1, 1989·The American Journal of Chinese Medicine·Y YoshimotoO Tanizawa
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Jul 15, 1994·Biochemical and Biophysical Research Communications·T SawadaA Miyake
Jul 15, 1994·Biochemical and Biophysical Research Communications·K KoikeA Miyake

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