PMID: 9535140Apr 16, 1998Paper

Evidence that human endothelial cells express different isoforms of Na,K-ATPase

Journal of Hypertension
V MayolJ M Maixent

Abstract

The catalytic alpha and smaller beta subunits of the plasma membrane Na,K-ATPase occur in various molecular forms (alpha1, alpha2, alpha3, beta1 and beta2). The alpha isoforms of the enzyme have varying affinities for ouabain and exist in different tissues with particular distribution patterns. To document the existence of isoforms of the Na,K-ATPase in cultured human umbilical vein endothelial cells. Microsomal fractions were prepared by differential ultracentrifugation from primary cultures of human umbilical vein endothelial cells and from such cells obtained after three passages. Na,K-ATPase activity was assayed using the coupled assay method and sensitivity to ouabain was determined in the presence of varying concentrations of ouabain. Specific antibodies for the various Na,K-ATPase isoforms were used to label these different proteins by immunocytochemistry in endothelial cells and by Western blotting in isolated membranes. In plotting the dose-response curves for Na,K-ATPase activity in response to ouabain we assumed the existence of two independent sites exhibiting different affinities for ouabain (in the micromol/l and the nmol/l ranges). The contribution of low-affinity sites was threefold that of high-affinity sites. ...Continue Reading

References

Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J M HamlynJ H Ludens
Jan 29, 1990·Biochimica Et Biophysica Acta·I Berrebi-BertrandL G Lelièvre
Jun 1, 1989·Circulation Research·C BoulangerP M Vanhoutte
Jan 1, 1994·Current Opinion in Nephrology and Hypertension·S W Graves
Mar 1, 1993·The American Journal of the Medical Sciences·J XieS S Greenberg

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Citations

Nov 16, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Hiroto MiuraDavid D Gutterman
Nov 22, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Barbara E StähliFelix C Tanner
May 13, 2017·Pflügers Archiv : European journal of physiology·Cristiana M L Di GiuroNicola Fameli

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