PMID: 3518633May 1, 1986

Evidence that isoniazid and ethanol induce the same microsomal cytochrome P-450 in rat liver, an isozyme homologous to rabbit liver cytochrome P-450 isozyme 3a

Archives of Biochemistry and Biophysics
D E RyanW Levin

Abstract

Cytochrome P-450j has been purified to electrophoretic homogeneity from hepatic microsomes of adult male rats administered ethanol and compared to the corresponding enzyme from isoniazid-treated rats. The enzymes isolated from ethanol- and isoniazid-treated rats have identical chromatographic properties, minimum molecular weights, spectral properties, peptide maps, NH2-terminal sequences, immunochemical reactivities, and substrate selectivities. Both preparations of cytochrome P-450j have high catalytic activity in aniline hydroxylation, butanol oxidation, and N-nitrosodimethylamine demethylation with turnover numbers of 17-18, 37-46, and 15 nmol product/min/nmol of P-450, respectively. A single immunoprecipitin band exhibiting complete identity was observed when the two preparations were tested by double diffusion analysis with antibody to isoniazid-inducible cytochrome P-450j. Ethanol- and isoniazid-inducible rat liver cytochrome P-450j preparations have also been compared and contrasted with cytochrome P-450 isozyme 3a, the major ethanol-inducible isozyme from rabbit liver. The rat and rabbit liver enzymes have slightly different minimum molecular weights and somewhat different peptide maps but similar spectral, catalytic, a...Continue Reading

References

Jul 1, 1979·Archives of Biochemistry and Biophysics·J S French, M J Coon
Apr 21, 1975·Biochemical and Biophysical Research Communications·J D Dignam, H W Strobel
Jun 7, 1976·Biochemical and Biophysical Research Communications·J P VilleneuveJ G Joly
Apr 30, 1985·Biochemical and Biophysical Research Communications·C S YangM J Coon
Sep 16, 1974·Biochimica Et Biophysica Acta·A Y Lu, W Levin
Sep 8, 1966·Biochemical and Biophysical Research Communications·N E Sladek, G J Mannering
Nov 15, 1981·Analytical Biochemistry·W WrayR Hancock
Oct 30, 1984·Biochemical and Biophysical Research Communications·M Ingelman-Sundberg, H Jörnvall
Nov 15, 1984·Archives of Biochemistry and Biophysics·D R KoopM J Coon
Jan 1, 1983·Pharmacology, Biochemistry, and Behavior·M J CoonE T Morgan
Nov 1, 1982·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·D E RyanW Levin
May 15, 1980·Analytical Biochemistry·M NumazawaT Nambara

Citations

Mar 1, 1988·Biochemical Pharmacology·G BánhegyiJ Mandl
Jan 1, 1990·Pharmacology & Therapeutics·D E Ryan, W Levin
Jan 1, 1990·Pharmacology & Therapeutics·C S Lieber
May 16, 1990·Biochimica Et Biophysica Acta·L Clejan, A I Cederbaum
Jul 21, 1992·Biochimica Et Biophysica Acta·D K Winters, A I Cederbaum
Apr 1, 1992·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·R P Gupta, M B Abou-Donia
Jan 1, 1990·Toxicology in Vitro : an International Journal Published in Association with BIBRA·D V ParkeD F Lewis
Jan 1, 1992·Free Radical Biology & Medicine·R NordmannH Rouach
Oct 21, 1991·Biochimica Et Biophysica Acta·S ImaokaY Funae
Jun 1, 1993·European Journal of Pharmacology·K H ParkB J Song
Nov 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·P H JellinckC P Martucci
Aug 30, 1988·Biochemical and Biophysical Research Communications·M T Williams, L C Simonet
Jan 4, 2001·Environmental Toxicology and Pharmacology·J AsteinzaJ J Espinosa-Aguirre
Feb 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S C KhaniM J Coon
Jan 1, 1991·Drug and Alcohol Review·C S Lieber
Feb 1, 1991·British Journal of Clinical Pharmacology·M M EpsteinJ M Wright
Jul 1, 1995·British Journal of Clinical Pharmacology·S WanwimolrukS Thitiarchakul
Dec 1, 1986·European Journal of Biochemistry·Y Terelius, M Ingelman-Sundberg
Oct 1, 1988·Annals of Human Genetics·P C BullI R Phillips
Aug 1, 1990·Alcoholism, Clinical and Experimental Research·D LucasJ F Menez
Feb 1, 1991·Alcoholism, Clinical and Experimental Research·K G IshakM B Ray
Aug 1, 1991·Alcoholism, Clinical and Experimental Research·C S Lieber
Dec 1, 1991·Alcoholism, Clinical and Experimental Research·E Dicker, A I Cederbaum
Jul 9, 1999·Alcoholism, Clinical and Experimental Research·C S Lieber
Nov 1, 1994·Environmental Health Perspectives·J A BondL Recio
Jan 1, 1988·Drug Metabolism and Drug Interactions·E H JefferyW M Haschek
Feb 25, 2014·Toxicology·Jessica H HartmanGunnar Boysen
Dec 19, 2013·Biochemical Pharmacology·Jessica H HartmanGrover P Miller
Aug 9, 2011·Annual Review of Pharmacology and Toxicology·Caroline H JohnsonFrank J Gonzalez
Apr 7, 2011·Archives of Pharmacal Research·Keumhan NohTae Cheon Jeong
Mar 27, 2010·Arhiv za higijenu rada i toksikologiju·Seema Sharma, Suresh Vir Singh Rana
Nov 12, 2005·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Michael FairheadGianfranco Gilardi
Mar 12, 2004·Journal of Ethnopharmacology·Yun-Hee Shon, Kyung-Soo Nam
Mar 7, 2002·Toxicology Letters·Soo J OhYoung C Kim
Jun 30, 1987·Toxicology and Applied Pharmacology·J M Fraser, L S Kaminsky
Jan 1, 1989·Drug Metabolism Reviews·R G Thurman, J A Handler
Jan 1, 1989·Drug Metabolism Reviews·J B SchenkmanL V Favreau
Jan 1, 1989·Drug Metabolism Reviews·D R KoopD J Tierney
Dec 1, 1994·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·V Nedelcheva, I Gut
Mar 1, 1993·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·K A Bachmann, L Jauregui
Feb 1, 1993·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·A C JapengaB G Lake
Mar 1, 1990·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·H IshizakiC S Yang
Jul 1, 1991·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·J H Fentem, J R Fry
Nov 8, 2001·The Journal of Pharmacy and Pharmacology·P P Maliakal, S Wanwimolruk
Sep 27, 2005·The International Journal of Biochemistry & Cell Biology·Terrence M DonohueDahn L Clemens
Dec 24, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S Krähenbühl
Mar 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D Wu, A I Cederbaum
Jan 1, 1993·Critical Reviews in Toxicology·J RaucyJ M Lasker
Jan 1, 1986·Alcoholism, Clinical and Experimental Research·D R Koop, M J Coon
Oct 14, 1987·Biochemical and Biophysical Research Communications·J M LaskerC S Lieber
May 1, 1993·Toxicology and Industrial Health·V LongoP G Gervasi
Dec 1, 1990·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D F WuA I Cederbaum
Dec 1, 1993·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M TsutsumiA Takada
May 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S Schenker, W C Maddrey
Oct 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M YamauchiG Toda
Nov 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Y C ChaoC T Hsu
Oct 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M TsutsumiC S Lieber
Sep 1, 1996·Clinical Pharmacology and Therapeutics·J T SlatteryK E Thummel
Oct 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T CastilloH Tsukamoto
Dec 1, 1991·Journal of the American College of Nutrition·C S Lieber
Dec 22, 1988·The New England Journal of Medicine·C S Lieber
Dec 1, 1995·Anesthesia and Analgesia·E D Kharasch
Jan 1, 1988·Critical Reviews in Toxicology·L S Kaminsky, J M Fraser
Oct 6, 2006·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Frank J Gonzalez

Related Concepts

Aniline diphosphate (4: 1)
Ethanol
Butanols
Aniline Compounds
Metazoa
Cytochrome P-450 Oxygenase
Enzyme Induction
Hydrolysis
Hydroxylation
IgG2B

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