Evidence that leptin contributes to intestinal cholesterol absorption in obese (ob/ob) mice and wild-type mice

Lipids
M IgelBergmann K von

Abstract

In the present study, the effect of leptin on intestinal cholesterol absorption was investigated in C57 BL/6 OlaHsd Lep(ob)/Lep(ob) obese (ob/ob) mice and lean C57 BL/6 (wild-type) mice. Animals were treated either with or without recombinant leptin for 2 wk. Cholesterol absorption was measured by the constant isotope feeding method and indirectly by the ratio of campesterol to cholesterol in serum. In ob/ob mice, cholesterol absorption was significantly higher compared to wild-type mice [83.4 +/- 2.3% (SD) vs. 77.6 +/- 1.5%, P < 0.01]. Treatment with leptin significantly reduced cholesterol absorption in both ob/ob and wild-type mice by 8.5 (P < 0.001) and 5.2% (P < 0.05), respectively. Serum concentrations of campesterol and the ratio of campesterol to cholesterol in ob/ob mice were significantly higher compared to wild-type mice (2.2 +/- 0.3 mg/dL vs. 1.2 +/- 0.3 mg/dL, P< 0.001; and 36.8 +/- 2.8 microg/mg vs. 28.0 +/- 3.3 microg/mg, P < 0.001). After treatment of ob/ob mice with leptin, concentrations of campesterol and its ratio to cholesterol were significantly lower (2.2 +/- 0.3 mg/dL vs. 1.0 +/- 0.2 microg/mg, P < 0.001; and 36.8 +/- 2.8 microg/mg vs. 13.2 +/- 2.2 microg/mg, P < 0.001, respectively). In wild-type mice, ...Continue Reading

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Citations

Feb 28, 2013·The British Journal of Nutrition·Lotta K StenmanRiitta Korpela
Feb 1, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Ronghua YangLili A Barouch
Jul 14, 2010·Progress in Histochemistry and Cytochemistry·Philippe G CammisottoMoise Bendayan
Apr 15, 2004·Biochemical and Biophysical Research Communications·Jaione BarrenetxeM Pilar Lostao
Mar 21, 2003·Metabolism: Clinical and Experimental·H VierhapperC Bieglmayer
Apr 27, 2012·Anatomy & Cell Biology·Philippe Cammisotto, Moise Bendayan

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