Evidence that masking of synapsis imperfections counterbalances quality control to promote efficient meiosis

PLoS Genetics
Susanna Mlynarczyk-EvansAnne M Villeneuve

Abstract

Reduction in ploidy to generate haploid gametes during sexual reproduction is accomplished by the specialized cell division program of meiosis. Pairing between homologous chromosomes and assembly of the synaptonemal complex at their interface (synapsis) represent intermediate steps in the meiotic program that are essential to form crossover recombination-based linkages between homologs, which in turn enable segregation of the homologs to opposite poles at the meiosis I division. Here, we challenge the mechanisms of pairing and synapsis during C. elegans meiosis by disrupting the normal 1:1 correspondence between homologs through karyotype manipulation. Using a combination of cytological tools, including S-phase labeling to specifically identify X chromosome territories in highly synchronous cohorts of nuclei and 3D rendering to visualize meiotic chromosome structures and organization, our analysis of trisomic (triplo-X) and polyploid meiosis provides insight into the principles governing pairing and synapsis and how the meiotic program is "wired" to maximize successful sexual reproduction. We show that chromosomes sort into homologous groups regardless of chromosome number, then preferentially achieve pairwise synapsis during a...Continue Reading

References

Dec 5, 1974·Journal of Molecular Biology·H F EpsteinS Brenner
Jan 1, 1984·Annual Review of Genetics·D von WettsteinP B Holm
Jan 1, 1980·Molecular & General Genetics : MGG·S S Siddiqui, P Babu
Feb 28, 1998·Science·K S McKimR S Hawley
Jun 4, 1999·Trends in Genetics : TIG·O Hobert, G Ruvkun
Feb 26, 2000·Annual Review of Genetics·D Zickler, N Kleckner
Jan 1, 2004·Nature Genetics·Christopher J BeanWilliam G Kelly
Feb 8, 2006·PLoS Genetics·Peter M CarltonAbby F Dernburg
Mar 9, 2006·Chromosoma·Esther de Boer, Christa Heyting
Apr 24, 2007·Trends in Genetics : TIG·Edward E Large, Laura D Mathies
Jun 30, 2007·Developmental Biology·Aimee Jaramillo-LambertJoAnne Engebrecht
Feb 4, 2009·Nature Reviews. Genetics·Paul S BurgoyneJames M A Turner
Jun 16, 2009·Nature Cell Biology·Sarah M Wignall, Anne M Villeneuve
Jul 22, 2009·Nature Cell Biology·Carolyn M PhillipsAbby F Dernburg
Nov 20, 2009·Developmental Cell·Yasushi Hiraoka, Abby F Dernburg
Jul 28, 2010·Developmental Biology·Dyan E MorganJudith Kimble
Jan 11, 2012·The Journal of Cell Biology·David J WynneAbby F Dernburg
Feb 15, 2012·Proceedings of the National Academy of Sciences of the United States of America·Adele AdamoAdriana La Volpe

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy
fluorescence imaging
PCR
dissection

Software Mentioned

GraphPad InStat
Adobe Photoshop
Prism
softWoRx
Volocity

Related Concepts

Related Feeds

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

Related Papers

Trends in Cell Biology
Wojciech P Pawlowski, W Zacheus Cande
Current Opinion in Genetics & Development
Enrique Martinez-Perez, Mónica P Colaiácovo
Advances in Experimental Medicine and Biology
Doris Y Lui, Mónica P Colaiácovo
© 2021 Meta ULC. All rights reserved