PMID: 6111398Jan 1, 1981Paper

Evidence that microtubule depolymerization early in the cell cycle is sufficient to initiate DNA synthesis

Cell
K L Crossin, D H Carney

Abstract

Microtubule disrupting drugs initiated DNA synthesis in serum-free cultures of nonproliferating fibroblast-like cells. The addition of colchicine to chick, mouse and human fibroblasts in serum-free medium stimulated thymidine incorporation at least twofold, with a half-maximal concentration of 1 X 10(-7) M. This stimulation represented up to 75% of the maximal stimulation by thrombin and was paralleled by an increase in the percentage of labeled nuclei. Other microtubule disrupting drugs showed similar stimulation, whereas lumicolchicine had no effect. Indirect immunofluorescent staining of tubulin showed a correlation between microtubule depolymerization and initiation of DNA synthesis by these drugs. A 2 hr treatment with 10(-6) M colchicine caused complete disruption of the microtubular network and stimulated thymidine incorporation (measured 28 hr later) to an even greater extent than continuous colchicine exposure. A similar 2 hr exposure to 10(-6) M colcemid also stimulated thymidine incorporation and led to a 50% increase in cell number. Taxol, a drug which stabilizes cytoplasmic microtubules, blocks initiation of DNA synthesis by colchicine, indicating that microtubule depolymerization is necessary for this initiation. ...Continue Reading

References

Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·B R BrinkleyD P Highfield
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·G M Edelman, I Yahara
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D A McClainG M Edelman
Nov 1, 1977·Cell·M Osborn, K Weber
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·Y ShechterP Cuatrecasas
Jun 21, 1979·Nature·J L GoldsteinM S Brown
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·Y ShechterP Cuatrecasas
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A M OttoL Jimenez de Asua
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J B BakerD D Cunningham
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M FriedkinE Rozengurt
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J De MeyG Geuens
Feb 22, 1979·Nature·P B SchiffS B Horwitz
Aug 18, 1977·Nature·D H Carney, D D Cunningham
Apr 1, 1978·Journal of Cellular Physiology·D H CarneyD D Cunningham
Jan 1, 1978·Journal of Supramolecular Structure·D H Carney, D D Cunningham
Jan 1, 1976·Journal of Supramolecular Structure·G M Fuller, B R Brinkley
Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·B Bhattacharyya, J Wolff
Jun 30, 1975·Annals of the New York Academy of Sciences·L Wilson
May 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·J M VasilievV I Guelstein
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P B Schiff, S B Horwitz
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D A McClain, G M Edelman

❮ Previous
Next ❯

Citations

Nov 1, 1996·Microscopy Research and Technique·M L MillerT Cody
Dec 1, 1994·Journal of Cellular Biochemistry·J MacoskaK J Pienta
Jan 1, 1987·Cancer Metastasis Reviews·A Raz, A Ben-Ze'ev
Mar 6, 2007·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Akihiro NakajoTakashi Aikou
Jan 1, 1983·Developmental Biology·B H Devlin, I R Konigsberg
Dec 1, 1981·Journal of Steroid Biochemistry·G A PucaV Sica
Jan 1, 1991·Life Sciences·J P Wiebe, C J Dinsdale
Jan 1, 1984·Pharmacology & Therapeutics·J J Manfredi, S B Horwitz
Oct 1, 1991·Pharmacology & Therapeutics·E K Rowinsky, R C Donehower
Nov 1, 1989·Trends in Neurosciences·C D Rasmussen, A R Means
Jul 10, 1991·Biochimica Et Biophysica Acta·Y AnrakuH Iida
Jan 1, 1985·Biochimica Et Biophysica Acta·J M Vasiliev
Nov 1, 1992·Trends in Cardiovascular Medicine·C L GraverA R Means
Feb 27, 1999·Current Opinion in Cell Biology·G G Gundersen, T A Cook
Jul 8, 2000·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·V Bourgarel-ReyC Briand
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J M Vasiliev, I M Gelfand
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J B Smith
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·I N ChouE Rapaport
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·K L Crossin
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·L A KrushelK L Crossin
Mar 21, 2001·Biotechnology & Genetic Engineering Reviews·D J Durzan
Sep 1, 1986·The Journal of Cell Biology·R L BallW C Thompson
Nov 1, 1994·The Journal of Cell Biology·P R Andreassen, R L Margolis
May 1, 1982·The Journal of Cell Biology·P Sherline, R N Mascardo
Sep 1, 1984·The Journal of Cell Biology·P F Turner, R L Margolis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
M FriedkinE Rozengurt
Proceedings of the National Academy of Sciences of the United States of America
A M OttoL Jimenez de Asua
Proceedings of the National Academy of Sciences of the United States of America
J M VasilievV I Guelstein
Proceedings of the National Academy of Sciences of the United States of America
A A ReszkaE H Fischer
© 2021 Meta ULC. All rights reserved