Evidence that P36, a human sperm acrosomal antigen involved in the fertilization process is triosephosphate isomerase

Molecular Reproduction and Development
Jana AuerM De Almeida

Abstract

P36 is one of the immunodominant sperm antigens identified by antibodies eluted from the spermatozoa of infertile men. In a previous study, we isolated and characterized this auto-antigen as a glycoprotein with several isoforms. Specific rabbit antibodies were produced to investigate sperm topography and the role of P36 in the fertilization process and we showed that P36 is present on the equatorial segment of acrosome-reacted spermatozoa and is involved in sperm-binding and the penetration of zona-free hamster oocytes. In the present study, we demonstrated, by means of immunofluorescence and electron microscopy, that P36 is present all over the acrosomal membranes of non-reacted spermatozoa. We also investigated the role of P36 in the acrosome reaction and sperm binding to the zona pellucida (ZP). The exposure of capacitated spermatozoa to rabbit anti-P36 antibodies had no effect on primary fixation to the ZP, but inhibited secondary binding to the ZP and the Ca2+ ionophore-induced acrosome reaction. These results suggest that P36, an acrosomal antigen, is involved in several steps of the fertilization process. On two-dimensional Western blots, human anti-sperm antibodies (ASA) and rabbit anti-P36 antibodies recognized five to...Continue Reading

References

Jun 1, 1994·Molecular Reproduction and Development·A M CourtotS J Weinman
Mar 1, 1996·Analytical Chemistry·A ShevchenkoM Mann
Jul 1, 1996·Journal of Reproduction and Fertility·S A CoonrodM E Westhusin
May 8, 1998·Human Reproduction·J W GraczykowskiR Z Sokol
Aug 12, 1998·Memórias do Instituto Oswaldo Cruz·S C BourguignonS G De Simone
Apr 25, 2001·Biology of Reproduction·C J FlickingerJ C Herr
Jun 21, 2001·Biochemical and Biophysical Research Communications·R K Naz, X Zhu
Jun 23, 2001·Molecular Human Reproduction·M RattanachaiyanontN Tanphaichitr

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Citations

Jun 13, 2008·Journal de la Société de biologie·Catherine SerresPierre Jouannet
Jan 29, 2013·Human Reproduction·F M PetitJ Auer
Jul 12, 2007·Seminars in Immunopathology·L W Chamley, G N Clarke
Apr 26, 2016·Journal of Human Reproductive Sciences·Mohammad-Sadegh Soltani ZangbarBehrouz Gharesi-Fard
Jul 24, 2013·Molecular Reproduction and Development·Takashi W IjiriGeorge L Gerton
Aug 1, 2012·Reproduction in Domestic Animals = Zuchthygiene·C Mukai, A J Travis
Aug 1, 2012·Reproduction in Domestic Animals = Zuchthygiene·C M Kershaw-Young, W M C Maxwell
Nov 18, 2014·Biochemical Society Transactions·François M PetitJana Auer
May 3, 2013·Systems Biology in Reproductive Medicine·Bahar BehrouziClifford L Librach
Sep 26, 2009·Chemistry & Biology·Chinatsu MukaiAlexander J Travis
Jul 20, 2014·Reproductive Sciences·Angela WagnerLawrence W Chamley
Dec 6, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Constance J Jeffery
Nov 30, 2006·Molecular Reproduction and Development·Jennifer J VendittiBarry S Bean
Nov 9, 2017·Science Progress·Constance J Jeffery
May 28, 2019·Molecular Reproduction and Development·Olivier D'AmoursRobert Sullivan

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