Abstract
Zellweger fibroblasts, which are devoid of peroxisomes and fail to synthesize plasmalogens, are very sensitive to the killing effect triggered by UV-activated 12-(1-pyrene) dodecanoic acid (P12). Although in some studied performed, it is assumed that reactive oxygen species (ROS) may damage plasma membrane causing necrosis, other studies suggest that ROS are involved in apoptotic cell death induced by a wide variety of stimuli. Analysing the P12 dose-response in Zellweger fibroblasts, we observed that at high doses (1-2 microM), more than 75% of the cells died after 24 h. This behaviour suggested that, at high doses, P12 kills the cells by unspecific lytic mechanisms or by necrosis, while at low doses (0.1-0.5 microM), an apoptotic mechanism could be involved. Cytofluorimetric analysis of Zellweger fibroblasts-treated with activated P12 (0.5 microM) did not show morphological modifications typical of apoptotic cell death. This was supported by comparative staining of fibroblast nuclei, DNA gel electrophoresis and identification of poly(ADP-ribose) polymerase (PARP) cleavage and Bcl-2 expression, assayed by Western blots. Thus, our results, while confirming the importance of plasmalogens in the protection against ROS, establish ...Continue Reading
References
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G A KellerR A Hallewell
Oct 21, 1991·Biochimica Et Biophysica Acta·E A WiemerJ M Tager
Feb 3, 1992·Biochimica Et Biophysica Acta·C DiveA H Wyllie
Mar 7, 1991·Nature·T TsukamotoY Fujiki
Dec 1, 1991·The Journal of Clinical Investigation·G HoeflerH W Moser
Jan 1, 1991·Cell and Tissue Research·T MasudaH D Fahimi
Nov 1, 1990·Nature·K TanakaY Okada
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A W SchramJ M Tager
Jun 1, 1988·The Journal of Clinical Investigation·S BrulJ M Tager
May 1, 1985·The Journal of Cell Biology·J A AriasS L Goldfischer
Oct 22, 1993·Cell·D M HockenberyS J Korsmeyer
Aug 1, 1995·Experimental Cell Research·E SpisniV Tomasi
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·N CartierO Danos
Mar 10, 1995·Science·C B Thompson
May 1, 1994·Analytical Biochemistry·J GongZ Darzynkiewicz
May 18, 1994·Biochimica Et Biophysica Acta·R Ofman, R J Wanders
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·W D JarvisS Grant
Jan 1, 1993·Cytometry·K A BlackG J Smith
Jan 1, 1993·Biochimie·H van den BoschR B Schutgens
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·A De SarioG Bernardi
Jun 1, 1996·The Journal of Cell Biology·M WeilM C Raff
Jun 19, 1997·Nature·J C Reed
Citations
Jul 9, 2004·Histochemistry and Cell Biology·Michael Schrader, H Dariush Fahimi
Jul 18, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Andrzej SurdackiJürgen C Frölich
Jul 5, 2011·Chemistry and Physics of Lipids·Stefan Wallner, Gerd Schmitz
Oct 5, 2001·The American Journal of Pathology·E BaumgartM Baes
Oct 13, 2006·Biochimica Et Biophysica Acta·Michael Schrader, H Dariush Fahimi