PMID: 9551086Apr 29, 1998Paper

Evidence that photodynamic stress kills Zellweger fibroblasts by a nonapoptotic mechanism

Biochimica Et Biophysica Acta
E SpisniV Tomasi

Abstract

Zellweger fibroblasts, which are devoid of peroxisomes and fail to synthesize plasmalogens, are very sensitive to the killing effect triggered by UV-activated 12-(1-pyrene) dodecanoic acid (P12). Although in some studied performed, it is assumed that reactive oxygen species (ROS) may damage plasma membrane causing necrosis, other studies suggest that ROS are involved in apoptotic cell death induced by a wide variety of stimuli. Analysing the P12 dose-response in Zellweger fibroblasts, we observed that at high doses (1-2 microM), more than 75% of the cells died after 24 h. This behaviour suggested that, at high doses, P12 kills the cells by unspecific lytic mechanisms or by necrosis, while at low doses (0.1-0.5 microM), an apoptotic mechanism could be involved. Cytofluorimetric analysis of Zellweger fibroblasts-treated with activated P12 (0.5 microM) did not show morphological modifications typical of apoptotic cell death. This was supported by comparative staining of fibroblast nuclei, DNA gel electrophoresis and identification of poly(ADP-ribose) polymerase (PARP) cleavage and Bcl-2 expression, assayed by Western blots. Thus, our results, while confirming the importance of plasmalogens in the protection against ROS, establish ...Continue Reading

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Citations

Jul 9, 2004·Histochemistry and Cell Biology·Michael Schrader, H Dariush Fahimi
Jul 18, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Andrzej SurdackiJürgen C Frölich
Jul 5, 2011·Chemistry and Physics of Lipids·Stefan Wallner, Gerd Schmitz
Oct 13, 2006·Biochimica Et Biophysica Acta·Michael Schrader, H Dariush Fahimi

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