Evidence that "pinin", reportedly a differentiation-specific desmosomal protein, is actually a widespread nuclear protein

Differentiation; Research in Biological Diversity
J M BrandnerW W Franke

Abstract

A protein recently described as a desmosome-specific molecule involved in anchoring intermediate-sized filaments (IFs) to the desmosomal plaque, and hence named "pinin" [43], has been known in our laboratory for several years as a strictly nuclear protein occurring in a wide range of cell types, including many that are totally devoid of desmosomes. Using a series of specific antibodies we have localized the protein in the nucleoplasm of cultured cells, blood cells and solid tissues of diverse vertebrate species, from oocytes to erythrocytes of amphibia and from liver to connective tissue and fibroblasts in mammals. Desmosomes have consistently been negative, and the nuclear specificity of the immunolocalization reactions has also been directly demonstrated by double-label immunofluorescence microscopy. From our results we conclude that this nuclear protein, characterized by a domain exceptionally rich in serine residues and hence termed DRS-protein, occurs in at least two genetically different forms in a diffusible state as well as in special ribonucleoprotein-particles, "speckles" [6], and is a widespread if not ubiquitous nuclear protein. Consequently it must serve nuclear functions rather than "pinning" IFs to plasma membran...Continue Reading

References

Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J O Thomas, R D Kornberg
Sep 1, 1992·International Journal of Peptide and Protein Research·M SchnölzerS B Kent
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P J KochW W Franke
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D L Spector
Jan 1, 1990·Annual Review of Cell Biology·M A SchwarzW W Franke
Oct 1, 1985·The Journal of Cell Biology·P CowinW W Franke
Dec 1, 1985·The Journal of Cell Biology·S Tsukita, S Tsukita
Mar 1, 1988·The Journal of Cell Biology·P BoukampN E Fusenig
Oct 1, 1989·The Journal of Cell Biology·Y HiedaS Tsukita
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·W W FrankeP Cowin
Jan 1, 1974·International Review of Cytology·L A Staehelin
Jan 1, 1982·Differentiation; Research in Biological Diversity·W W FrankeH Mueller
Oct 1, 1994·Current Opinion in Cell Biology·P J Koch, W W Franke
Dec 1, 1994·Differentiation; Research in Biological Diversity·H W HeidW W Franke
Aug 1, 1993·Molecular Biology Reports·A I Lamond, M Carmo-Fonseca
Jan 1, 1993·Annual Review of Cell Biology·D L Spector
Feb 1, 1993·The Journal of Cell Biology·E Shtivelman, J M Bishop
Feb 1, 1993·Current Opinion in Cell Biology·D R Garrod
May 1, 1993·The Journal of Cell Biology·R S BuxtonJ R Stanley
Feb 1, 1997·The Journal of Investigative Dermatology·I MollW W Franke

❮ Previous
Next ❯

Citations

Mar 26, 2011·Histochemistry and Cell Biology·Shu-Yuan HsuPin Ouyang
Mar 1, 2012·Histochemistry and Cell Biology·Stefan Hübner, Athina Efthymiadis
Apr 25, 2012·Histochemistry and Cell Biology·Shu-Yuan HsuPin Ouyang
Jun 1, 2004·Molecular Biology of the Cell·Noriko SaitohDavid L Spector
Jan 20, 2004·Molecular and Cellular Biology·Eiji SakashitaAkila Mayeda
Nov 25, 2011·Critical Reviews in Eukaryotic Gene Expression·Catherine D Mao, Stephen W Byers
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·J Eilbracht, M S Schmidt-Zachmann
Feb 6, 2002·The Journal of Cell Biology·Paula Sacco-Bubulya, David L Spector
Jan 24, 2006·Gene Expression Patterns : GEP·Steve Leu, Pin Ouyang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.