Evidence that protein kinase C is involved in delta-aminolevulinate synthase expression in rat hepatocytes

Archives of Biochemistry and Biophysics
C L Varone, E T Canépa

Abstract

There are many factors that regulate the rate of synthesis of delta-aminolevulinate synthase (ALA-S), the enzyme which governs the rate-limiting step in heme biosynthesis. In rat hepatocytes, phenobarbital increases ALA-S gene transcription and dibutyryl cAMP potentiates this induction, whereas insulin and glucose have the opposite effect. The present report provides evidence that protein kinase C (PKC) activation negatively influences ALA-S mRNA levels, as measured by Northern and slot-blot analysis. The addition of 1,2-dioctanoyl-sn-glycerol (DOG) or 12-O-tetradecanoylphorbol 13-acetate (TPA), a PKC activator that mimics diacylglycerol function, to cultures led to a significant decrease of both basal and phenobarbital-induced ALA-S mRNA levels in a dose-dependent manner. This TPA effect depends on the specific activation of PKC because the analog 4 alpha-phorbol 12,13-diacetate, a nonstimulatory PKC phorbol ester, is unable to inhibit ALA-S mRNA. Furthermore, the effect of TPA is blocked by the PKC inhibitors staurosporine and calphostin C. Desensitization of the PKC pathway by prolonged exposure to TPA abolished the subsequent action of the phorbol ester. On the other hand, neither TPA nor DOG modified the half-life of ALA-S...Continue Reading

References

Apr 1, 1976·Analytical Biochemistry·J R FryJ W Bridges
Jan 1, 1992·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·E T CánepaM Grinstein
Sep 15, 1992·European Journal of Biochemistry·A AzziC Hensey
Jun 1, 1992·Experimental Cell Research·J L Messina, R S Weinstock
Aug 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R M O'BrienD K Granner
Apr 15, 1991·Biochemical and Biophysical Research Communications·R F BrunsH Nakano
Jun 1, 1990·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·E T CánepaM Grinstein
Oct 1, 1989·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J L Hargrove, F H Schmidt
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R A Galbraith, A Kappas
Jul 18, 1986·Science·Y Nishizuka
Aug 15, 1988·Biochemical and Biophysical Research Communications·M Wolf, M Baggiolini
Jan 1, 1986·Current Topics in Cellular Regulation·B K MayW H Elliott
Jun 1, 1984·Molecular and Cellular Endocrinology·B Koepfer-Hobelsberger, O H Wieland
Sep 1, 1994·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·C L VaroneM Grinstein
Oct 1, 1993·The International Journal of Biochemistry·M R Moore
Apr 15, 1993·The Biochemical Journal·H Hug, T F Sarre

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Citations

Mar 29, 2014·Biochimica Et Biophysica Acta·Silvina V SonzogniEduardo T Cánepa
Apr 6, 2012·Molecular and Cellular Biochemistry·Abel L CarcagnoEduardo T Cánepa
Sep 11, 2014·Molecular and Cellular Biochemistry·Silvina V SonzogniEduardo T Cánepa
Dec 22, 1999·Archives of Biochemistry and Biophysics·C L VaroneE T Cánepa
Mar 31, 2005·Archives of Biochemistry and Biophysics·Alejandra S GubermanEduardo T Cánepa

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