PMID: 6103496Apr 1, 1980Paper

Evidence that somatostatin inhibits proinsulin synthesis and insulin release by isolated pancreatic islets of the rat

Metabolism: Clinical and Experimental
F G Williams, G E Bauer

Abstract

Conflicting reports on the direction and magnitude of the effect of somatostatin on (pro)insulin synthesis prompted our investigation. Two assays for proinsulin synthesis were designed in which [4,5-3H]-L-leucine incorporation into proinsulin was normalized on the basis of postincubation insulin levels rather than on the number of islets incubated. Somatostatin at a concentration of 10 micrograms/ml inhibited 300 mg/dl glucose-stimulated proinsulin synthesis by 25% from 448 +/- 25 dpm/microunits insulin to 336 +/- 25 dpm/microunits insulin (disintegrations per minute in the proinsulin peak per microunit extractable insulin) (p less than 0.05). Glucagon (10 micrograms/ml) reversed the inhibitory effect of somatostatin on proinsulin synthesis from 336 +/- 25 dpm/microunits insulin to 480 +/- 44 dpm/microunits insulin (p less than 0.02). Somatostatin (10 micrograms/ml) had no significant effect on proinsulin synthesis in the presence of 70 mg/dl or 150 mg/dl glucose. Insulin release in 300 mg/dl glucose was inhibited 38% by 10 micrograms/ml somatostatin from 3.05 +/- 0.40 mU medium/mU tissue to 1.90 +/- 0.10 mU medium/mU tissue (p less than 0.01) over a 45-minute incubation period. These data suggest that somatostatin may act on g...Continue Reading

References

Jan 1, 1976·Proceedings of the Society for Experimental Biology and Medicine·N M Baird, G E Bauer
Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L OrciR H Unger
Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M P Dubois
Jan 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D L Curry, L L Bennett
Jan 1, 1976·Life Sciences·J A Zivin, J J Bartko
Aug 1, 1969·Endocrinology·A LindallR Sorenson
Oct 8, 1974·Biochemical and Biophysical Research Communications·D L Curry, L L Bennett
Sep 7, 1967·Biochemical and Biophysical Research Communications·J R Turtle, D M Kipnis

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