Evidence that some oligodendrocyte progenitors in the developing optic pathway express the plp gene

Glia
M L FanarragaI R Griffiths

Abstract

DM-20, a product of the proteolipid protein (plp) gene, has been demonstrated in the spinal cord of the mouse embryo as early as embryonic day 12 (E12) in certain cells, some of which are identifiable as oligodendrocyte progenitors. The present work uses optic pathways of rat and mouse as well-characterized systems for the study of gliogenesis. plp gene expression was monitored with a combination of reverse transcriptase polymerase chain reaction, in situ hybridization, and immunostaining with antibodies to different PLP peptide sequences, combined with O-2A lineage markers. In tissue sections, hybridizing cells were detected initially in the proximal optic tracts between E18 and birth and thereafter progressively in the chiasm and optic nerves. Small unbranched cells expressing DM-20 but not myelin basic protein (MBP) and probably representing progenitors were detectable by immunostaining in similar locations. With increasing postnatal ages, cells representing maturing oligodendrocytes which co-label for PLP and MBP are present in the optic pathways. In vitro analysis of freshly dissociated cells from premyelinated optic nerve demonstrated that the plp gene is expressed in some O-2A progenitor cells as well as mature oligodend...Continue Reading

Citations

Jul 22, 1998·Microscopy Research and Technique·I GriffithsK A Nave
Jul 27, 1999·Neuropathology and Applied Neurobiology·R H Woodruff, R J Franklin
May 26, 1998·Brain Research. Brain Research Reviews·J M VelaB Castellano
Nov 18, 2005·Molecular and Cellular Neurosciences·Jörn SchweitzerHauke Werner

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