PMID: 9450930Feb 28, 1998Paper

Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae

Genes & Development
G A HartzogF Winston

Abstract

Previous characterization of the Saccharomyces cerevisiae Spt4, Spt5, and Spt6 proteins suggested that these proteins act as transcription factors that modify chromatin structure. In this work, we report new genetic and biochemical studies of Spt4, Spt5, and Spt6 that reveal a role for these factors in transcription elongation. We have isolated conditional mutations in SPT5 that can be suppressed in an allele-specific manner by mutations in the two largest subunits of RNA polymerase II (Pol II). Strikingly, one of these RNA Pol II mutants is defective for transcription elongation and the others cause phenotypes consistent with an elongation defect. In addition, we show that spt4, spt5, and spt6 mutants themselves have phenotypes suggesting defects in transcription elongation in vivo. Consistent with these findings, we show that Spt5 is physically associated with RNA Pol II in vivo, and have identified a region of sequence similarity between Spt5 and NusG, an Escherichia coli transcription elongation factor that binds directly to RNA polymerase. Finally, we show that Spt4 and Spt5 are tightly associated in a complex that does not contain Spt6. These results, taken together with the biochemical identification of a human Spt4-Spt5...Continue Reading

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Citations

Mar 17, 2004·Biochimica Et Biophysica Acta·Jane Mellor, Antonin Morillon
Mar 17, 2004·Biochimica Et Biophysica Acta·Ali Shilatifard
Oct 11, 2003·Trends in Genetics : TIG·Karen M Arndt, Caroline M Kane
Sep 6, 2002·Biochimica Et Biophysica Acta·Grant A HartzogDerek L Lindstrom
Sep 6, 2002·Biochimica Et Biophysica Acta·Rachel N Fish, Caroline M Kane
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Apr 7, 2011·The Journal of Biological Chemistry·Olga V ViktorovskayaDavid A Schneider
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Jun 5, 2003·Genes & Development·Chwen-Huey WuDavid Gilmour

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