Evidence that the action of cholecystokinin octapeptide on the guinea pig ileum longitudinal muscle is mediated in part by substance P release from the myenteric plexus

European Journal of Pharmacology
J B Hutchison, G J Dockray

Abstract

Studies were made on the mode of action of cholecystokinin octapeptide (CCK8) on longitudinal muscle strips of guinea pig ileum in vitro. CCK8 produced dose-related contractions of innervated strips of ileum longitudinal muscle (EC50 3--4 x 10(-9) M), but was inactive on denervated strips. Tetrodotoxin, and reducing the bath temperature to 15 degree C, abolished the response of innervated strips to CCK8. These results indicate that the action of CCK8 on ileal longitudinal muscle is mediated by the myenteric plexus. Atropine abolished the responses to acetylcholine and to low doses of CCK8. However, the responses to CCK8 in doses above 10(-8) M were reduced by atropine but not abolished. Incubation of longitudinal muscle strips with high concentrations of substance P (1.5 x 10(-7) M) resulted in desensitization to this peptide; this treatment inhibited the responses to CCK8 alone, and abolished the atropine-resistant actions of CCK8. Desensitization to substance P had no effect on the responses to acetylcholine, histamine, serotonin and angiotensin II. The results indicate that CCK8 contracts the longitudinal muscle of guinea pig ileum by releasing acetylcholine and substance P from the myenteric plexus.

References

May 15, 1977·European Journal of Pharmacology·A A WaterfieldG Henderson
Nov 30, 1979·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·Y KatayamaJ T Williams
Apr 30, 1979·Naunyn-Schmiedeberg's Archives of Pharmacology·R FrancoJ B Furness
Jul 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J E MullerR S Yalow
May 28, 1979·Naunyn-Schmiedeberg's Archives of Pharmacology·R FrancoJ B Furness
Sep 1, 1977·Clinical and Experimental Pharmacology & Physiology·R W Bury, M L Mashford
Jan 1, 1975·Histochemistry·A G Pearse, J M Polak
Jan 1, 1975·Histochemistry·G NilssonF Sundler
Nov 1, 1975·Acta Physiologica Scandinavica·P Hedqvist, U S von Euler
May 1, 1970·The Journal of Physiology·N AmbacheM A Zar
Apr 10, 1980·Nature·T HökfeltM Schultzberg
Feb 27, 1953·The Journal of Physiology·J H GADDUM

❮ Previous
Next ❯

Citations

Jan 1, 1985·Naunyn-Schmiedeberg's Archives of Pharmacology·J DonnererF Lembeck
Jun 16, 1982·European Journal of Pharmacology·J P Huidobro-ToroJ M Musacchio
May 4, 1984·European Journal of Pharmacology·A W Mangel
Oct 22, 1985·European Journal of Pharmacology·S L CohenT N Chase
Oct 22, 1985·European Journal of Pharmacology·P R NemethJ D Wood
Jan 10, 1989·European Journal of Pharmacology·G J Sanger, D R Nelson
Jun 13, 1996·European Journal of Pharmacology·G Varga, C Scarpignato
Jan 1, 1995·Life Sciences·M T MartínE Goñalons
Aug 1, 1991·Journal of the Autonomic Nervous System·M ShirahataS Lahiri
Jan 1, 1993·Journal of Physiology, Paris·C ScarpignatoC Corradi
May 7, 2004·Peptides·Véronique LerayJean-Paul Galmiche
Jan 1, 1997·Pharmacology & Therapeutics·P Holzer, U Holzer-Petsche
May 1, 1990·British Journal of Pharmacology·M D'AmatoA Bennett
Feb 14, 2006·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·S R MartinsG Ballejo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.