PMID: 9639568Jun 26, 1998Paper

Evidence that the conformation of unliganded human plasminogen is maintained via an intramolecular interaction between the lysine-binding site of kringle 5 and the N-terminal peptide

The Biochemical Journal
C S CockellC P Ponting

Abstract

Human Glu-plasminogen adopts at least three conformations that provide a means for regulating the specificity of its activation in vivo. It has been proposed previously that the closed (alpha) conformation of human Glu-plasminogen is maintained through physical interaction of the kringle 5 domain and a lysine residue within the N-terminal peptide (NTP). To examine this hypothesis, site-directed mutagenesis was used to generate variant proteins containing substitutions either for aspartic acid residues within the anionic centre of the kringle 5 domain or for conserved lysine residues within the NTP. Size-exclusion HPLC and rates of plasminogen activation by urokinase-type plasminogen activator were used to determine the conformational states of these variants. Variants with substitutions within the kringle 5 lysine-binding site demonstrated extended conformations, as did variants with alanine substitutions for Lys50 and Lys62. In contrast, molecules in which NTP residues Lys20 or Lys33 were replaced were shown to adopt closed conformations. We conclude that the lysine-binding site of kringle 5 is involved in maintaining the closed conformation of human Glu-plasminogen via an interaction with the NTP, probably through Lys50 and/o...Continue Reading

Citations

Jul 29, 2008·The Journal of Biological Chemistry·Ingrid M Verhamme, Paul E Bock
Apr 19, 2012·The Journal of Antibiotics·Haruki KoideKeiji Hasumi
May 3, 2014·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Keiji KoyanagiKeiji Hasumi
Aug 23, 2016·Acta Ophthalmologica·Claudia Kuhli-HattenbachLars-Olof Hattenbach
Sep 22, 1999·European Journal of Biochemistry·J A KornblattC Balny
Feb 5, 2003·The Journal of Biological Chemistry·Lev BeckerMarlys L Koschinsky
May 19, 2006·Journal of Thrombosis and Haemostasis : JTH·M C DrinaneM J Mulligan-Kehoe
Aug 8, 2007·Biochemistry. Biokhimii︠a︡·M Yu LevashovS D Varfolomeyev
Nov 24, 2011·The Journal of Antibiotics·Haruki KoideKeiji Hasumi
Sep 16, 2010·The Journal of Antibiotics·Keiko HasegawaKeiji Hasumi
Mar 13, 2015·Biomedit︠s︡inskai︠a︡ khimii︠a︡·A A TykhomyrovT V Grinenko
May 12, 2017·International Journal of Peptide Research and Therapeutics·Maciej PurwinKrystyna Midura-Nowaczek
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ermanno GherardiDmitri I Svergun
Dec 7, 2007·The Journal of Antibiotics·Masaaki SasaokaKeiji Hasumi
Aug 21, 2014·The Journal of Biological Chemistry·Ingrid M Verhamme, Paul E Bock
May 1, 2012·Journal of Thrombosis and Haemostasis : JTH·Y XueK Olsson
Jan 9, 2007·Biotechnology Letters·Zhifeng MaJian-Ning Liu
Nov 18, 2009·Biochemistry. Biokhimii︠a︡·R B AisinaS D Varfolomeyev
Dec 10, 2009·Blood·Tiphaine DejouvencelEduardo Anglés-Cano
Jan 13, 2012·Chembiochem : a European Journal of Chemical Biology·Joakim E Swedberg, Jonathan M Harris
Oct 2, 2007·Arthritis and Rheumatism·Mary Jo Mulligan-KehoeMichael Simons
Dec 10, 2015·Journal of Lipid Research·Michael B Boffa, Marlys L Koschinsky
Feb 8, 2020·Haematologica·Claire S Whyte, Nicola J Mutch
Jan 23, 2021·International Journal of Molecular Sciences·Keiji Hasumi, Eriko Suzuki
Jul 10, 2007·Biochemistry·Inna P GladyshevaGuy L Reed
May 15, 2002·Biochimica Et Biophysica Acta·Tadashi Kikuchi, Keiji Hasumi
Jan 5, 2014·Biochemistry·Yogesh KumarS Paul Bajaj
Feb 26, 2008·Biochemical and Biophysical Research Communications·Moyuru HayashiMotoyuki Shimonaka

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