Evidence that the FK506-binding immunophilin heat shock protein 56 is required for trafficking of the glucocorticoid receptor from the cytoplasm to the nucleus

Molecular Endocrinology
M J CzarW B Pratt

Abstract

The FK506-binding immunophilin hsp56 (FKBP52) is one of several chaperone proteins associated with untrasformed steroid receptors in a multiprotein heterocomplex. The function of heat shock protein 56 (hsp56) with respect to receptor action is unknown. hsp56 is not required for glucocorticoid receptor heterocomplex assembly or for proper folding of the receptor hormone-binding domain into a high affinity steroid-binding conformation. In intact cells, the majority of the hsp56 is located in the nucleus, with a minority colocalizing with microtubules in the cytoplasm. hsp56 contains a conserved negatively charged domain that we speculate might serve as a nuclear localization signal recognition sequence. Here we show that injection of an antibody raised against this negative sequence into intact L cells impedes subsequent dexamethasone-mediated shift of the glucocorticoid receptor into the nucleus. Nonimmune rabbit serum and an antibody raised against another site on hsp56 do not affect receptor movement. Inhibition of receptor movement by the 419 antibody against the negative sequence is blocked by preincubation with purified hsp56, but not by preincubation with purified hsp90, hsp70, or BSA. These observations are consistent wit...Continue Reading

Citations

May 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Beili WuZihe Rao
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·Y MiyataE E Baulieu
Oct 28, 2011·The Journal of Biological Chemistry·Yeshavanth K Banasavadi-SiddegowdaXiaodong Wang
Mar 22, 2001·Molecular and Cellular Biology·A KazlauskasI Pongratz
Mar 11, 2008·Investigative Ophthalmology & Visual Science·Xinyu ZhangThomas Yorio
Jun 30, 2009·PloS One·Rudolph ReimerWolfgang Bohn
Oct 16, 2001·Cell Stress & Chaperones·M HaverinenT Ylikomi
Mar 21, 1998·Insect Biochemistry and Molecular Biology·Q SongL I Gilbert
May 9, 2012·Cellular and Molecular Life Sciences : CMLS·Alexandra Kollara, Theodore J Brown
Feb 12, 1998·The Journal of Steroid Biochemistry and Molecular Biology·C M KlingeR Hilf
Oct 23, 1998·Invertebrate Neuroscience : in·L I GilbertR Rybczynski
Oct 1, 1996·Plant Molecular Biology·R S BostonE Vierling
Apr 1, 1999·Cell Biochemistry and Biophysics·D B DeFranco
Apr 15, 2008·Trends in Endocrinology and Metabolism : TEM·D O Toft
Jul 14, 2007·Molecular and Cellular Endocrinology·Iwona Grad, Didier Picard
Sep 29, 2011·Traffic·Sofie VandevyverClaude Libert
Sep 24, 2004·The International Journal of Biochemistry & Cell Biology·Todd H Davies, Edwin R Sánchez
Sep 12, 2015·Gene·Michael Haase, Guido Fitze
May 12, 2006·Molecular and Cellular Endocrinology·Lily VardimonPazit Polak
May 2, 2008·Journal of Immunological Methods·Guillermo J CopelloLuis E Díaz
Jan 5, 2002·Journal of Virology·Jan-Jong HungWen Chang
Oct 16, 2004·Molecular and Cellular Biology·Joëlle RüeggTheo Rein

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