Evidence that the integrin beta3 and beta5 subunits contain a metal ion-dependent adhesion site-like motif but lack an I domain.

The Journal of Biological Chemistry
E C LinJ W Smith

Abstract

The amino-terminal domain of each integrin beta subunit is hypothesized to contain an ion binding site that is key to cell adhesion. A new hypothesis regarding the structure of this site is suggested by the crystallization of the I domains of the integrin alphaL and alphaM subunits (Lee, J.-O., Rieu, P., Arnaout, M. A., and Liddington, R. (1995) Cell 80, 631-638; Qu, A., and Leahy, D. J. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 10277-10281). In those proteins, an essential metal ion is bound by a metal ion-dependent adhesion site (MIDAS). The MIDAS is presented at the apex of a larger protein module called an I domain. The metal ligands in the MIDAS can be separated into three distantly spaced clusters of oxygenated residues. These three coordination sites also appear to exist in the integrin beta3 and beta5 subunits. Here, we examined the putative metal binding site within beta3 and beta5 using site-directed mutagenesis and ligand binding studies. We also investigated the fold of the domain containing the putative metal binding site using the PHD structural algorithm. The results of the study point to the similarity between the integrin beta subunits and the MIDAS motif at two of three key coordination points. Importantly th...Continue Reading

References

Mar 1, 1991·The Journal of Cell Biology·C A PraterW A Frazier
Aug 1, 1988·Cell Structure and Function·T YatohgoM Hayashi
Nov 1, 1989·Trends in Neurosciences·A PersechiniR H Kretsinger
Dec 1, 1989·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M A Horton, J Davies
Oct 23, 1987·Science·E Ruoslahti, M D Pierschbacher
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·A Qu, D J Leahy
Dec 1, 1995·Science·M FriedlanderD A Cheresh
Apr 22, 1994·Science·P C BrooksD A Cheresh
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·C F BarbasJ W Smith
Jul 20, 1993·Journal of Molecular Biology·B Rost, C Sander
Jan 19, 1996·The Journal of Biological Chemistry·A J PelletierV Quaranta
Jun 1, 1996·Methods : a Companion to Methods in Enzymology·M Malmqvist
Feb 1, 1994·Trends in Cell Biology·G R NemerowT J Wickham

❮ Previous
Next ❯

Citations

Feb 22, 2000·The Journal of Biological Chemistry·J L BanèresJ Parello
May 10, 2000·The Journal of Biological Chemistry·E F PlowJ W Smith
Feb 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tetsuji KamataYoshikazu Takada
Oct 14, 2004·The Journal of Cell Biology·DeannaLee M BeauvaisAlan C Rapraeger
May 26, 2006·The Journal of Biological Chemistry·Michelle M PeshoEdward F Plow
Sep 20, 1997·The Journal of Biological Chemistry·E C LinJ W Smith
Mar 30, 2001·The Journal of Biological Chemistry·Y M Xiong, L Zhang
Nov 19, 2011·Journal of Cellular Biochemistry·Joel Raborn, Bing-Hao Luo
Jun 6, 2000·The Journal of Biological Chemistry·J M HigginsM B Brenner
Jun 25, 2014·The Journal of Biological Chemistry·Maciej J StawikowskiGregg B Fields
Jun 5, 2001·The Journal of Biological Chemistry·A P CoeM J Humphries
Sep 18, 2002·Immunological Reviews·M Amin Arnaout
Jan 18, 2002·The Journal of Biological Chemistry·Aleksandra Cierniewska-CieslakEdward F Plow
Aug 10, 1999·Experimental Cell Research·D L BrassardL Armstrong
May 14, 1999·Immunology Today·M E Binnerts, Y van Kooyk
Dec 8, 2000·Biochimica Et Biophysica Acta·B LeitingerN Hogg
Sep 17, 2002·Current Opinion in Cell Biology·M Amin ArnaoutJian-Ping Xiong
Dec 24, 1997·Matrix Biology : Journal of the International Society for Matrix Biology·J W Smith
May 17, 2003·Experimental Cell Research·DeannaLee M Beauvais, Alan C Rapraeger
Mar 6, 2002·Progress in Histochemistry and Cytochemistry·Donald E Gullberg, Evy Lundgren-Akerlund

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.