PMID: 9190804Jun 1, 1997Paper

Evidence that the N-terminal part of the S-layer protein from Bacillus stearothermophilus PV72/p2 recognizes a secondary cell wall polymer

Journal of Bacteriology
W RiesMargit Sára

Abstract

The S-layer of Bacillus stearothermophilus PV72/p2 shows oblique lattice symmetry and is composed of identical protein subunits with a molecular weight of 97,000. The isolated S-layer subunits could bind and recrystallize into the oblique lattice on native peptidoglycan-containing sacculi which consist of peptidoglycan of the A1gamma chemotype and a secondary cell wall polymer with an estimated molecular weight of 24,000. The secondary cell wall polymer could be completely extracted from peptidoglycan-containing sacculi with 48% HF, indicating the presence of phosphodiester linkages between the polymer chains and the peptidoglycan backbone. The cell wall polymer was composed mainly of GlcNAc and ManNAc in a molar ratio of 4:1, constituted about 20% of the peptidoglycan-containing sacculus dry weight, and was also detected in the fraction of the S-layer self-assembly products. Extraction experiments and recrystallization of the whole S-layer protein and proteolytic cleavage fragments confirmed that the secondary cell wall polymer is responsible for anchoring the S-layer subunits by the N-terminal part to the peptidoglycan-containing sacculi. In addition to this binding function, the cell wall polymer was found to influence the i...Continue Reading

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Citations

Jul 25, 2006·Bioscience, Biotechnology, and Biochemistry·Guangshan ZhaoKazuo Sakka
Dec 25, 2010·Physical Chemistry Chemical Physics : PCCP·Christian ZafiuWolfgang Kautek
Jun 22, 2011·The Journal of Biological Chemistry·Christine HorejsDietmar Pum
Apr 25, 2012·Extremophiles : Life Under Extreme Conditions·Federico AcostaJosé Berenguer
Jun 13, 2013·Small·Judith Ferner-Ortner-BleckmannUwe B Sleytr
Apr 23, 2013·Small·Mehmet H UcisikUwe B Sleytr
Feb 4, 2014·FEMS Microbiology Reviews·Uwe B SleytrDietmar Pum
May 9, 2014·Journal of the Royal Society, Interface·Bernhard Schuster, Uwe B Sleytr
Aug 28, 2016·FEMS Microbiology Reviews·Christina Schäffer, Paul Messner
May 17, 2013·Applied Microbiology and Biotechnology·Ulla Hynönen, Airi Palva
Nov 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Dieter MollMargit Sára
Mar 1, 2007·FEMS Microbiology Letters·Uwe B SleytrEva M Egelseer
Mar 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Kelly C Rice, Kenneth W Bayles
Feb 15, 2000·Journal of Bacteriology·E Hoiczyk, A Hansel
Jan 29, 2000·Journal of Bacteriology·M Sára, U B Sleytr
Jul 26, 2005·Applied Microbiology and Biotechnology·Guangshan ZhaoKazuo Sakka
May 24, 2005·Infection and Immunity·Yukie ShibataYoshihisa Yamashita

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