Evidence that the primary binding site of von Willebrand factor that mediates platelet adhesion on subendothelium is not collagen

The Journal of Clinical Investigation
P G De GrootJ J Sixma

Abstract

We have studied the binding of von Willebrand factor to extracellular matrices of endothelial cells and to the vessel wall of human umbilical arteries in relation to its function in supporting platelet adhesion. CLB-RAg 201, an MAb against von Willebrand factor, completely inhibits the binding of von Willebrand factor to collagen type I and type III. CLB-RAg 201 does not inhibit the binding of 125I-von Willebrand factor to extracellular matrices of endothelial cells, to smooth muscle cells, or to the subendothelium. CLB-RAg 201 partly inhibits platelet adhesion to these surfaces, but this directly affects the interaction between von Willebrand factor and platelets and is not due to inhibition of binding of von Willebrand factor to these surfaces. Another MAb, CLB-RAg 38, does not inhibit the binding of von Willebrand factor to collagen. CLB-RAg 38 completely inhibits the binding of von Willebrand factor to extracellular matrices. CLB-RAg 38 inhibits platelet adhesion to cellular matrices completely insofar as it is dependent on plasma von Willebrand factor. CLB-RAg 38 does not inhibit the total binding of von Willebrand factor to subendothelium, as there are too many different binding sites, but it completely inhibits the funct...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Nov 27, 1979·Biochemistry·H SageP Bornstein
Nov 1, 1978·Thrombosis Research·Y J LegrandJ P Caen
Feb 28, 1978·Biochemical and Biophysical Research Communications·P J Fraker, J C Speck
Oct 16, 1978·Biochemical and Biophysical Research Communications·M J BarnesC I Levene
Aug 19, 1976·The New England Journal of Medicine·R Ross, J A Glomset
Dec 22, 1970·Biochimica Et Biophysica Acta·J A Van Mourik, I A Mochtar
Jul 1, 1983·The Journal of Clinical Investigation·Z M RuggeriR R Montgomery
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·Z M RuggeriL de Marco
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D D WagnerV J Marder
Aug 1, 1980·Thrombosis Research·K S SakariassenJ J Sixma

Citations

Sep 15, 1995·Thrombosis Research·M ZangariJ H Rand
Jul 3, 1995·Biochimica Et Biophysica Acta·D BhattacharyaP C Mandal
Aug 7, 2002·Blood Reviews·John Pimanda, Philip Hogg
May 7, 2013·Annual Review of Biomedical Engineering·Thomas V ColaceScott L Diamond
Oct 6, 1998·Annual Review of Biochemistry·J E Sadler
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Brian SavageZ M Ruggeri
May 1, 1991·Mayo Clinic Proceedings·D MeyerJ P Girma
Jan 1, 1996·Progress in Histochemistry and Cytochemistry·H SuzukiK Tanoue
Apr 1, 1990·Transfusion Medicine Reviews·T J Kunicki
Jan 1, 1986·Critical Reviews in Oncology/hematology·R J HamerJ J Sixma
Jan 24, 2015·Hämostaseologie·Z M Ruggeri, G L Mendolicchio
Jul 1, 1989·Baillière's Clinical Haematology·D BaruchD Meyer
Oct 1, 1989·Baillière's Clinical Haematology·H Pannekoek, J Voorberg
Mar 23, 2010·Translational Research : the Journal of Laboratory and Clinical Medicine·Alessandra CasonatoAntonio Pagnan
Jul 1, 1990·British Journal of Haematology·P G De Groot, J J Sixma
Apr 23, 2004·Journal of Thrombosis and Haemostasis : JTH·R W FarndaleP G De Groot
Jan 1, 1996·Arteriosclerosis, Thrombosis, and Vascular Biology·J J SixmaP G De Groot
Mar 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·C DenisD Meyer

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