Evidence that the promoter can influence assembly of antitermination complexes at downstream RNA sites

Journal of Bacteriology
Ying ZhouDavid I Friedman

Abstract

The N protein of phage lambda acts with Escherichia coli Nus proteins at RNA sites, NUT, to modify RNA polymerase (RNAP) to a form that overrides transcription terminators. These interactions have been thought to be the primary determinants of the effectiveness of N-mediated antitermination. We present evidence that the associated promoter, in this case the lambda early P(R) promoter, can influence N-mediated modification of RNAP even though modification occurs at a site (NUTR) located downstream of the intervening cro gene. As predicted by genetic analysis and confirmed by in vivo transcription studies, a combination of two mutations in P(R), at positions -14 and -45 (yielding P(R-GA)), reduces effectiveness of N modification, while an additional mutation at position -30 (yielding P(R-GCA)) suppresses this effect. In vivo, the level of P(R-GA)-directed transcription was twice as great as the wild-type level, while transcription directed by P(R-GCA) was the same as that directed by the wild-type promoter. However, the rate of open complex formation at P(R-GA) in vitro was roughly one-third the rate for wild-type P(R). We ascribe this apparent discrepancy to an effect of the mutations in P(R-GCA) on promoter clearance. Based on ...Continue Reading

References

Jan 1, 1979·Annual Review of Genetics·M Rosenberg, D Court
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·A DasS Adhya
Feb 1, 1992·Journal of Bacteriology·S L SullivanM E Gottesman
Mar 1, 1991·Journal of Bacteriology·H MoyleM M Susskind
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·B C Hoopes, W R McClure
Jan 1, 1985·Annual Review of Biochemistry·W R McClure
Jul 25, 1983·Journal of Molecular Biology·D F WardM E Gottesman
Jan 1, 1980·Annual Review of Genetics·I Herskowitz, D Hagen
Mar 1, 1996·Journal of Molecular Biology·T Kubori, N Shimamoto
Aug 1, 1996·Molecular Microbiology·A T SchauerD I Friedman
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·J W RobertsC W Roberts
Feb 7, 2001·The Journal of Biological Chemistry·R SenN Shimamoto
May 17, 2000·Proceedings of the National Academy of Sciences of the United States of America·D YuD L Court
Apr 3, 2001·Current Opinion in Microbiology·D I Friedman, D L Court
May 25, 2002·Journal of Bacteriology·Ying ZhouDavid I Friedman
Aug 9, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Evgeny Nudler, Max E Gottesman
Nov 14, 2002·Annual Review of Genetics·Donald L CourtLynn C Thomason
Dec 4, 2002·Environmental Microbiology·Julia HoltzendorffWolfgang R Hess
Dec 13, 2002·The Journal of Biological Chemistry·Michael CashelV James Hernandez
May 6, 2003·Science·Vitaly Epshtein, Evgeny Nudler
Sep 13, 2003·The EMBO Journal·Vitaly EpshteinEvgeny Nudler
Jan 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yvonne Berghöfer-HochheimerCarol A Gross
Feb 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Oren KobilerAmos B Oppenheim

❮ Previous
Next ❯

Citations

Aug 2, 2007·Journal of Applied Genetics·Agnieszka Szalewska-PalaszAlicja Wegrzyn
May 23, 2015·Microbiology·Karl-Gustav RueggebergAnthony G Hay

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.