Evidence that the V kappa III gene usage is nonstochastic in both adult and newborn peripheral B cells and that peripheral CD5+ adult B cells are oligoclonal

The Journal of Clinical Investigation
J C WeberJ L Pasquali

Abstract

There is evidence that in certain situations the expressed antibody repertoire is dominated by small subsets of V gene segments. They include fetal, CD5+, and autoantibody-forming B cells as well as low grade B cell malignancies. For instance, inside the V kappa III family of approximately 10 members, only 3 (humkv325, 328, and Vg) are used recurrently for autoantibody production. However, the significance of this recurrence is difficult to interpret without a clear vision of the actual repertoire in normal subjects. To address this, we have sequenced and compared two sets of rearranged V kappa III genes generated by cDNA PCR amplification from a normal newborn, a normal adult, and from CD5+ B cells of the same adult donor. The results show that: (a) only four V kappa III gene segments are used by neonatal and total adult B cells (humkv325, humkv328, Vg, and kv305), humkv325 being overexpressed in both repertoires; (b) there is no significant difference in terms of V kappa III gene usage between the adult and newborn repertoires; (c) regarding the junction regions, there is a favored use of the most 5' JK gene segments (Jk1-Jk2); approximately 20% of the newborn and adult junction sequences was characterized by one or two addit...Continue Reading

References

Nov 11, 1992·Proceedings of the National Academy of Sciences of the United States of America·C F BarbasD R Burton
Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S L ZebedeeR A Lerner
Apr 1, 1992·Current Opinion in Immunology·R R Hardy
Apr 1, 1992·The Journal of Experimental Medicine·T MartinT J Kipps
May 1, 1991·European Journal of Immunology·M E SchutteT Logtenberg
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·R R Hardy, K Hayakawa
Nov 1, 1991·Immunology Today·A B Kantor
Jan 1, 1992·Arthritis and Rheumatism·V Pascual, J D Capra
Jun 1, 1991·The Journal of Experimental Medicine·H GuI Förster
Aug 1, 1991·European Journal of Immunology·W PargentH G Zachau
Aug 1, 1990·European Journal of Immunology·A MeindlH G Zachau
Feb 1, 1991·The Journal of Clinical Investigation·D A CarsonT J Kipps
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H W Schroeder, J Y Wang
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T J KippsD A Carson
Dec 1, 1986·The Journal of Experimental Medicine·V RadouxD A Carson
Oct 1, 1986·Immunological Reviews·R R Hardy, K Hayakawa
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T J KippsD A Carson
Nov 6, 1987·Science·H W SchroederR M Perlmutter
Dec 1, 1987·The Journal of Experimental Medicine·P P ChenD A Carson
Nov 20, 1987·Science·F W AltG D Yancopoulos
Dec 21, 1984·Nucleic Acids Research·M Pech, H G Zachau
Jun 1, 1993·European Journal of Immunology·S B EbelingT Logtenberg
Feb 1, 1993·The Journal of Experimental Medicine·A K StewartR S Schwartz

❮ Previous
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Citations

Nov 9, 2000·European Journal of Immunology·K D GeigerR Küppers
Jun 5, 2002·Molecular Immunology·Hermann J Girschick, Peter E Lipsky
May 29, 1998·Immunological Reviews·U KleinR Küppers
Apr 1, 1997·The Journal of Clinical Investigation·S J FosterP E Lipsky
Jul 27, 2011·Immunogenetics·Katherine Jean Louise JacksonAndrew M Collins
Oct 7, 2017·Current Allergy and Asthma Reports·María Belén Almejun, Mercedes Borge
Oct 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W G AbbottP L Tan
Nov 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·J LeeP E Lipsky
Jun 6, 1998·Clinical Immunology and Immunopathology·X WuD C Young

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