Abstract
Rat parotid acinar cells lacking zymogen granules were obtained by inducing granule discharge with the beta-adrenoceptor agonist isoproterenol. To assess whether zymogen granules are involved in the regulation of Ca(2+) signalling as intracellular Ca(2+) stores, changes in cytosolic free Ca(2+) ion concentration ([Ca(2+)](i)) were studied with imaging microscopy in fura-2-loaded parotid acinar cells lacking zymogen granules. The increase in [Ca(2+)](i) induced by muscarinic receptor stimulation was initiated at the apical pole of the acinar cells, and rapidly spread as a Ca(2+) wave towards the basolateral region. The magnitude of the [Ca(2+)](i) response and the speed of the Ca(2+) wave were essentially similar to those in control acinar cells containing zymogen granules. Western blot analysis of the inositol 1,4,5-trisphosphate receptor (IP(3)R) was performed on zymogen granule membranes and microsomes using anti-IP(3)R antibodies. The immunoreactivity of all three IP(3)Rs was clearly observed in the microsomal preparations. Although a weak band of IP(3)R type-2 was detected in the zymogen granule membranes, this band probably resulted from contamination by the endoplasmic reticulum (ER), because calnexin, a marker protein of...Continue Reading
References
Apr 1, 1975·The Journal of Cell Biology·F Clemente, J Meldolesi
Jan 1, 1992·Biology of the Cell·G NicaiseE Keicher
Dec 1, 1991·The Journal of Membrane Biology·A Marty
Dec 20, 1990·Nature·H Kasai, G J Augustine
Dec 10, 1990·Biochimica Et Biophysica Acta·A TanimuraY Tojyo
Feb 1, 1972·Archives of Oral Biology·R ShibaK Kawakatsu
Jun 1, 1969·The Journal of Cell Biology·A AmsterdamM Schramm
Aug 1, 1984·Pflügers Archiv : European journal of physiology·I NakagakiY Imai
Mar 22, 1994·Biochemistry·L W TjoelkerP W Gray
Jul 1, 1993·Nature·L G MészárosA Chu
Aug 27, 1993·Cell·P ThornO H Petersen
Aug 27, 1993·Cell·H KasaiY Miyashita
Feb 9, 1996·Cell·O V GerasimenkoO H Petersen
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·M RavazzolaL Orci
Apr 4, 1997·The Journal of Biological Chemistry·D I YuleR J Wojcikiewicz
Jun 20, 1997·The Journal of Biological Chemistry·D H DiJulioJ C Singh
Jun 20, 1997·The Journal of Biological Chemistry·M G LeeS Muallem
Jun 27, 1997·Cell·F IchasJ P Mazat
Nov 21, 1997·Biochemical and Biophysical Research Communications·Y TojyoY Matsumoto
Mar 21, 1998·Cell Calcium·Y TojyoY Matsumoto
Jun 8, 1999·The Journal of Biological Chemistry·T MurayamaY Ogawa
Jul 31, 1999·The Journal of Cell Biology·K ItoH Kasai
Aug 27, 1999·Cell Calcium·I MountianF Wuytack
Sep 16, 1999·The EMBO Journal·H TinelO H Petersen
Apr 25, 2000·The Journal of Biological Chemistry·S H YooC J Jeon
Jun 30, 2000·The Journal of Biological Chemistry·A TanimuraR J Turner
Mar 21, 2001·The Biochemical Journal·D K LoomsS Dissing
Citations
Apr 23, 2005·Cell and Tissue Research·Junko Fujita-YoshigakiHiroshi Sugiya
Feb 16, 2005·Annual Review of Physiology·James E MelvinTed Begenisich
Sep 16, 2003·American Journal of Respiratory Cell and Molecular Biology·Masami SonHiroaki Kume
Feb 3, 2009·Archives of Oral Biology·Akane ImaiHiromi Shimomura
Jul 2, 2011·Archives of Oral Biology·Takao MoritaYosuke Tojyo
Jan 3, 2012·Cell Calcium·Javier Alvarez
Nov 4, 2006·The Journal of Physiological Sciences : JPS·Yutaka EndoMasumi Inoue
Jun 7, 2006·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Akihiko Tanimura, Yosuke Tojyo