Evidences for a New Role of miR-214 in Chondrogenesis

Scientific Reports
V P RobertoD M Tiago

Abstract

miR-214 is known to play a role in mammalian skeletal development through inhibition of osteogenesis and stimulation of osteoclastogenesis, but data regarding other vertebrates, as well as a possible role in chondrogenesis, remain unknown. Here, we show that miR-214 expression is detected in bone and cartilage of zebrafish skeleton, and is downregulated during murine ATDC5 chondrocyte differentiation. Additionally, we observed a conservation of the transcriptional regulation of miR-214 primary transcript Dnm3os in vertebrates, being regulated by Ets1 in ATDC5 chondrogenic cells. Moreover, overexpression of miR-214 in vitro and in vivo mitigated chondrocyte differentiation probably by targeting activating transcription factor 4 (Atf4). Indeed, miR-214 overexpression in vivo hampered cranial cartilage formation of zebrafish and coincided with downregulation of atf4 and of the key chondrogenic players sox9 and col2a1. We show that miR-214 overexpression exerts a negative role in chondrogenesis by impacting on chondrocyte differentiation possibly through conserved mechanisms.

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Citations

Aug 5, 2018·Journal of Cellular Physiology·Youqiang SunJiake Xu
Jan 24, 2019·Annals of Biomedical Engineering·Md Tausif SalimAjit P Yoganathan
Jun 11, 2019·Molecular Medicine Reports·Hui ZhuYuping Tang
Nov 3, 2020·Epigenetics : Official Journal of the DNA Methylation Society·Shusaku AbeTakashi Ishiuchi
Jun 21, 2021·Biomaterials·Nuong Thi Kieu NguyenYu-Chen Hu

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Datasets Mentioned

BETA
KF774190

Methods Mentioned

BETA
PCR
ImmunoPrecipitation
ChIP
transfection
Assay

Software Mentioned

DIALIGN
UCSC Genome browser
GraphPad Prism
Ensembl genome browser
GraphPad
ConTra
RNAhybrid
CHAOS
Genomatix
MatInspector

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