Evidences of basal lactate production in the main white adipose tissue sites of rats. Effects of sex and a cafeteria diet

PloS One
Sofía ArriaránMarià Alemany

Abstract

Female and male adult Wistar rats were fed standard chow or a simplified cafeteria diet for one month. Then, the rats were killed and the white adipose tissue (WAT) in four sites: perigonadal, retroperitoneal, mesenteric and subcutaneous (inguinal) were sampled and frozen. The complete WAT weight in each site was measured. Gene expression analysis of key lipid and glucose metabolism enzymes were analyzed, as well as tissue and plasma lactate and the activity of lactate dehydrogenase. Lactate gradients between WAT and plasma were estimated. The influence of sex and diet (and indirectly WAT mass) on lactate levels and their relationships with lactate dehydrogenase activity and gene expressions were also measured. A main conclusion is the high production of lactate by WAT, practically irrespective of site, diet or sex. Lactate production is a direct correlate of lactate dehydrogenase activity in the tissue. Furthermore, lactate dehydrogenase activity is again directly correlated with the expression of the genes Ldha and Ldhb for this enzyme. In sum, the ability to produce lactate by WAT is not directly dependent of WAT metabolic state. We postulate that, in WAT, a main function of the lactate dehydrogenase path may be that of conv...Continue Reading

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Citations

Jul 7, 2018·Biopharmaceutics & Drug Disposition·Shuhei FukunoHiroki Konishi
Jan 17, 2020·Metabolites·Camila WeschenfelderAline Marcadenti
Oct 23, 2020·International Journal of Molecular Sciences·Xavier Remesar, Marià Alemany
Dec 24, 2020·International Journal of Molecular Sciences·Andjelika KalezicBato Korac

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Methods Mentioned

BETA
dissection
PCR
blood drawn

Software Mentioned

GraphPad
Statgraphics Centurion XVI
Prism

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