Evodiamine, a Novel NOTCH3 Methylation Stimulator, Significantly Suppresses Lung Carcinogenesis in Vitro and in Vivo

Frontiers in Pharmacology
Tao SuZhong-Qiu Liu

Abstract

Lung cancer is a leading cause of cancer-related deaths worldwide. NOTCH3 signaling is mainly expressed in non-small cell lung carcinoma (NSCLC), and has been proposed as a therapeutic target of NSCLC. While, few agents for preventing or treating NSCLC via targeting NOTCH3 signaling are used in modern clinical practice. Evodiamine (EVO), an alkaloid derived from Euodiae Fructus, possesses low toxicity and has long been shown to exert anti-lung cancer activity. However, the underlying anti-lung cancer mechanisms of EVO are not yet fully understood. In this study, we explored the involvement of NOTCH3 signaling in the anti-lung cancer effects of EVO. Urethane-induced lung cancer mouse model and two NSCLC cell models, A549 and H1299, were used to evaluate the in vivo and in vitro anti-lung cancer action of EVO. A DNA methyltransferase inhibitor was employed to investigate the role of NOTCH3 signaling in the anti-lung cancer effects of EVO. Results showed that EVO potently reduced tumor size and tumor numbers in mice, and inhibited NOTCH3 in the tumors. EVO also dramatically reduced cell viability, induced G2/M cell cycle arrest, inhibited cell migration and reduced stemness in cultured NSCLC cells. Mechanistic studies showed that ...Continue Reading

References

Apr 5, 2002·Nature·Ina RheeBert Vogelstein
Jun 4, 2002·Nature Reviews. Genetics·Peter A Jones, Stephen B Baylin
Apr 2, 2004·Biological & Pharmaceutical Bulletin·Masaru Ogasawara, Hideyo Suzuki
May 5, 2005·Journal of the National Cancer Institute·Federico CappuzzoMarileila Varella-Garcia
Jan 14, 2011·Journal of Neuro-oncology·Ganeshkumar RajendranAnjali Shiras
Jan 29, 2011·Digestion·Eiichiro YamamotoYasuhisa Shinomura
Jun 7, 2012·Methods in Molecular Biology·Karilyn E SantDana C Dolinoy
Jul 3, 2013·The Oncologist·Justin F Gainor, Alice T Shaw
Oct 22, 2013·Current Pharmaceutical Design·M CapellettiM Tiseo
Nov 10, 2013·Nature Medicine·Curtis R Chong, Pasi A Jänne
May 3, 2014·Cold Spring Harbor Perspectives in Biology·En Li, Yi Zhang
Oct 23, 2014·Journal of Cancer Prevention·Ji-Young HongSang Kook Lee
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Feb 6, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yanmei ZouHongge Wu
Nov 19, 2015·International Journal of Molecular Sciences·Lv-Cui ZhaoDi-Long Chen
Feb 4, 2016·Journal of Cancer Research and Clinical Oncology·Wael Abdo HassanTakaaki Ito
Jun 9, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Yuting LiuLinlin Lu
Oct 25, 2016·Advances in Experimental Medicine and Biology·Qunyou Tan, Jingqing Zhang
Dec 31, 2016·International Journal of Molecular Sciences·Catia GiovanniniLaura Gramantieri
Jan 14, 2017·Molecular Oncology·Kay T Yeung, Jing Yang
Oct 14, 2017·Journal of Molecular Histology·Fatma UysalGokhan Akkoyunlu

❮ Previous
Next ❯

Citations

Mar 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sun Tae HwangKwang Seok Ahn
Nov 6, 2018·International Journal of Molecular Sciences·Xing-Xian GuoJing Li
Nov 2, 2018·International Journal of Molecular Sciences·Xu HuFanxing Xu
Sep 26, 2019·Frontiers in Genetics·Sadia AkterTrupti Joshi
May 28, 2020·Journal of Hematology & Oncology·Yue YangLufeng Zheng
Dec 31, 2019·Seminars in Cancer Biology·Thomas Efferth, Franz Oesch
Nov 20, 2020·Journal of Experimental & Clinical Cancer Research : CR·Ze-Bo JiangElaine Lai-Han Leung
Jul 29, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Jian-Xia WenYan-Ling Zhao
Jul 31, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Saikat MitraJesus Simal-Gandara

❮ Previous
Next ❯

Methods Mentioned

BETA
light microscopy
Polymerase Chain Reaction
PCR
electrophoresis
MeDIP-Seq
FACS
flow cytometry
methylation profiling

Software Mentioned

GraphPad Prism
Image ProPlus
Quantity One
SPSS
MethHC
FlowJo
MassARRAY

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.