Evodiamine suppresses non-small cell lung cancer by elevating CD8+ T cells and downregulating the MUC1-C/PD-L1 axis.

Journal of Experimental & Clinical Cancer Research : CR
Ze-Bo JiangElaine Lai-Han Leung

Abstract

Accumulating evidence showed that regulating tumor microenvironment plays a vital role in improving antitumor efficiency. Programmed Death Ligand 1 (PD-L1) is expressed in many cancer cell types, while its binding partner Programmed Death 1 (PD1) is expressed in activated T cells and antigen-presenting cells. Whereas, its dysregulation in the microenvironment is poorly understood. In the present study, we confirmed that evodiamine downregulates MUC1-C, resulting in modulating PD-L1 expression in non-small cell lung cancer (NSCLC). Cell viability was measured by MTT assays. Apoptosis, cell cycle and surface PD-L1 expression on NSCLC cells were analyzed by flow cytometry. The expression of MUC1-C and PD-L1 mRNA was measured by real time RT-PCR methods. Protein expression was examined in evodiamine-treated NSCLC cells using immunoblotting or immunofluorescence assays. The effects of evodiamine treatment on NSCLC sensitivity towards T cells were investigated using human peripheral blood mononuclear cells and Jurkat, apoptosis and IL-2 secretion assays. Female H1975 xenograft nude mice were used to assess the effect of evodiamine on tumorigenesis in vivo. Lewis lung carcinoma model was used to investigate the therapeutic effects of ...Continue Reading

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Citations

Feb 5, 2021·Journal of Cellular and Molecular Medicine·Luis Del ValleZhiqiang Qin
May 9, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Ze-Bo JiangElaine Lai-Han Leung
Jul 31, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Saikat MitraJesus Simal-Gandara

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Methods Mentioned

BETA
glycosylation
transfection
flow cytometry
Assay
xenograft
xenografts

Software Mentioned

flowJo
Graph Pad Prism

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