Evolution of a site of specific genetic homology on the chromosome of Escherichia coli.

Journal of Bacteriology
P L Kahn

Abstract

Integration of the factors F(v) and F into the chromosome of a substrain of Escherichia coli K-12 has been studied. The F(v) factor is a fertility factor derived from Col V, lacking the ability to govern the production of colicin V. The derivatives of an Hfr(v) (Hfr isolated from a V colicinogenic parent) strain, PK2 (initially isolated from C600 V(+)), were shown to retain a unique bidirectional sex factor affinity locus between recA and pheA. This site shows no affinity for the E. coli K-12 F factor as shown by inability to isolate Hfr strains with origins in this region from a parental strain containing a cytoplasmic F factor. However this area exhibits two regions of homology to the V colicinogenic factor. One gives rise to Hfr(v) strains identical to the original Hfr(v) strain, PK2, with an origin and polarity of transfer designated pheA-CC injecting markers in the order pheA-his-trp-pro. The second gives rise to strains apparently originating at the same site but with reverse polarity designated recA-C, transferring markers in the order recA-thyA-str-xyl. For strains possessing the F(v) factor only the second homology is apparent. A model for the evolution of these strains is presented.

References

Dec 1, 1967·Bacteriological Reviews·A L Taylor, C D Trotter
Oct 1, 1967·Journal of Cellular Physiology·A J Clark
Feb 1, 1968·Journal of Bacteriology·E Dubnau, W K Maas
Dec 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·W Goebel, D R Helinski
Feb 14, 1969·Journal of Molecular Biology·C W HillP Berg
Oct 14, 1964·Biochemical and Biophysical Research Communications·T S MatneyR A Scroggs
Jan 1, 1966·Progress in Nucleic Acid Research and Molecular Biology·C A Thomas
Jul 1, 1966·Genetics·M DemerecP E Hartman

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