Evolution of alternative biosynthetic pathways for vitamin C following plastid acquisition in photosynthetic eukaryotes

ELife
Glen WheelerN Smirnoff

Abstract

Ascorbic acid (vitamin C) is an enzyme co-factor in eukaryotes that also plays a critical role in protecting photosynthetic eukaryotes against damaging reactive oxygen species derived from the chloroplast. Many animal lineages, including primates, have become ascorbate auxotrophs due to the loss of the terminal enzyme in their biosynthetic pathway, L-gulonolactone oxidase (GULO). The alternative pathways found in land plants and Euglena use a different terminal enzyme, L-galactonolactone dehydrogenase (GLDH). The evolutionary processes leading to these differing pathways and their contribution to the cellular roles of ascorbate remain unclear. Here we present molecular and biochemical evidence demonstrating that GULO was functionally replaced with GLDH in photosynthetic eukaryote lineages following plastid acquisition. GULO has therefore been lost repeatedly throughout eukaryote evolution. The formation of the alternative biosynthetic pathways in photosynthetic eukaryotes uncoupled ascorbate synthesis from hydrogen peroxide production and likely contributed to the rise of ascorbate as a major photoprotective antioxidant.

References

Jan 1, 1979·Journal of Nutritional Science and Vitaminology·S ShigeokaS Kitaoka
Apr 15, 1976·Nature·E C BirneyK M Ayaz
Mar 1, 1990·Biological Chemistry Hoppe-Seyler·K Dabrowski
Apr 1, 1986·Archives of Biochemistry and Biophysics·E Tel-OrL Packer
Jan 1, 1973·Annual Review of Entomology·R H Dadd
Dec 21, 1973·Science·I B Chatterjee
Nov 1, 1994·European Journal of Biochemistry·W K HuhS O Kang
Feb 26, 1996·FEBS Letters·G BánhegyiJ Mandl
Jun 10, 1998·Nature·G L WheelerN Smirnoff
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·P L ConklinR L Last
Dec 29, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·N Smirnoff
Jan 12, 2001·Vitamins and Hormones·N Smirnoff
Jul 19, 2003·Journal of Experimental Botany·Jeffrey Allan RunningAlan Berry
Sep 5, 2003·The Journal of Biological Chemistry·Beata A Wolucka, Marc Van Montagu
Jan 22, 2004·Nature Reviews. Genetics·Jeremy N TimmisWilliam Martin
Feb 21, 2004·Plant Physiology·Argelia LorenceCraig L Nessler
Oct 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Patrick J Keeling, Yuji Inagaki
Jan 14, 2005·Bioinformatics·Federico AbascalDavid Posada
Aug 10, 2005·Proceedings of the National Academy of Sciences of the United States of America·Shane R WilkinsonJohn M Kelly
Apr 6, 2006·The Journal of Biological Chemistry·Patricia L ConklinNicholas Smirnoff
Feb 1, 1982·Plant Physiology·J P HelsperF A Loewus
Nov 9, 2006·Bioscience, Biotechnology, and Biochemistry·Takahiro IshikawaShigeru Shigeoka
Jan 16, 2007·The FEBS Journal·Carole L Linster, Emile Van Schaftingen
Apr 5, 2007·Nature Protocols·Jan LisecAlisdair R Fernie
May 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·William A LaingSean M Bulley
Jun 26, 2007·The Plant Journal : for Cell and Molecular Biology·Christine OesterheltHeiko Lokstein
Sep 20, 2007·The Plant Journal : for Cell and Molecular Biology·John DowdleNicholas Smirnoff
Feb 19, 2008·Archives of Biochemistry and Biophysics·Nicole G H LeferinkWillem J H van Berkel
Sep 19, 2008·The Journal of Biological Chemistry·Bernard PineauRosine De Paepe
Oct 7, 2008·FEMS Microbiology Reviews·Amel LatifiCheng-Cai Zhang
Dec 18, 2008·The Journal of Biological Chemistry·Nicole G H LeferinkWillem J H van Berkel

❮ Previous
Next ❯

Citations

Dec 24, 2015·Plant Physiology and Biochemistry : PPB·Siddique I Aboobucker, Argelia Lorence
Apr 5, 2016·Current Opinion in Chemical Biology·Woodward W FischerJoan Selverstone Valentine
Apr 16, 2015·ELife·Alisdair R Fernie, Takayuki Tohge
Nov 23, 2017·Frontiers in Plant Science·Christophe LoixAnn Cuypers
Oct 5, 2017·Antioxidants & Redox Signaling·Szilvia Z TóthAndrás Szarka
Sep 18, 2018·The New Phytologist·Nicholas Smirnoff, Dominique Arnaud
Aug 14, 2018·Plant Biology·E Ivanov KavkovaR Tenhaken
Feb 9, 2019·Frontiers in Plant Science·Mario FenechMiguel A Botella
Nov 2, 2019·Antioxidants·Costantino PaciollaMaria Concetta de Pinto
Jul 2, 2020·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Konstantinos FeidantsisBasile Michaelidis
Mar 2, 2019·Annual Review of Plant Biology·Crysten E Blaby-Haas, Sabeeha S Merchant
Apr 4, 2020·Journal of Experimental Botany·Richard Raj SinghTina Kyndt
Jul 3, 2019·Frontiers in Plant Science·Riadh IlahyMarcello Salvatore Lenucci
Jul 27, 2017·Frontiers in Chemistry·Ifigeneia Mellidou, Angelos K Kanellis
Jul 30, 2020·Molecular Biology and Evolution·Juan David Bayona-SerranoInácio L M Junqueira-de-Azevedo
Dec 4, 2020·International Journal of Molecular Sciences·Rafe LyallTsanko Gechev
Mar 9, 2021·Frontiers in Plant Science·Dario Di SilvestreIrene Murgia
May 10, 2021·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Konstantinos FeidantsisBasile Michaelidis
Nov 5, 2015·Plant Physiology·Alisdair R Fernie, Eran Pichersky

❮ Previous
Next ❯

Datasets Mentioned

BETA
KJ957823

Methods Mentioned

BETA
PCR
Illumina sequencing

Software Mentioned

GBLOCKS
EGT
BLASTP
BEAST
ProtTest
TBLASTN
PhyML3
MEGA5
MUSCLE

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.