Evolution of an Escherichia coli protein with increased resistance to oxidative stress.

The Journal of Biological Chemistry
Z LuE C Lin

Abstract

L-1,2-Propanediol:NAD+ 1-oxidoreductase of Escherichia coli is encoded by the fucO gene, a member of the regulon specifying dissimilation of L-fucose. The enzyme normally functions during fermentative growth to regenerate NAD from NADH by reducing the metabolic intermediate L-lactaldehyde to propanediol which is excreted. During aerobic growth L-lactaldehyde is converted to L-lactate and thence to the central metabolite pyruvate. The wasteful excretion of propanediol is minimized by oxidative inactivation of the oxidoreductase, an Fe2+-dependent enzyme which is subject to metal-catalyzed oxidation (MCO). Mutants acquiring the ability to grow aerobically on propanediol as sole carbon and energy source can be readily selected. These mutants express the fucO gene constitutively, as a result of an IS5 insertion in the promoter region. In this study we show that continued selection for aerobic growth on propanediol resulted in mutations in the oxidoreductase conferring increased resistance to MCO. In two independent mutants, the resistance of the protein was respectively conferred by an Ile7 --> Leu and a Leu8 --> Val substitution near the NAD-binding consensus amino acid sequence. A site-directed mutant protein with both substituti...Continue Reading

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Citations

Mar 23, 2011·Applied Microbiology and Biotechnology·Satoko NiimiHideaki Yukawa
May 4, 2010·Applied and Environmental Microbiology·Dae-Hee Lee, Bernhard Ø Palsson
Oct 13, 2000·Journal of Bacteriology·C A Holland-StaleyP R Cunningham
Nov 10, 2010·PLoS Computational Biology·Dipak BaruaJennifer L Reed
Mar 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Pedro EchaveE C C Lin
Nov 23, 2006·Metabolic Engineering·Isabelle Meynial SallesPhilippe Soucaille
Jan 24, 2021·Microbial Cell Factories·Aditya Vikram PanditRadhakrishnan Mahadevan

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